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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Haplopelma hainanum HNTX-0314931 CSB-YP320597HUK
CSB-EP320597HUK
CSB-BP320597HUK
CSB-MP320597HUK
CSB-EP320597HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum HNTX-0314974 CSB-YP320598HUK
CSB-EP320598HUK
CSB-BP320598HUK
CSB-MP320598HUK
CSB-EP320598HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti HWTX-XI-IS5 CSB-YP320599HUL
CSB-EP320599HUL
CSB-BP320599HUL
CSB-MP320599HUL
CSB-EP320599HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma schmidti HWTX-XI-IS40 CSB-YP320600HUL
CSB-EP320600HUL
CSB-BP320600HUL
CSB-MP320600HUL
CSB-EP320600HUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Secreted effector protein EspF (U) CSB-YP320601EOD
CSB-EP320601EOD
CSB-BP320601EOD
CSB-MP320601EOD
CSB-EP320601EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translocated intimin receptor Tir (tir), partial CSB-YP320602ENL
CSB-EP320602ENL
CSB-BP320602ENL
CSB-MP320602ENL
CSB-EP320602ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cellulosilyticus Putative membrane protein Bcell_0381 (Bcell_0381), partial CSB-YP320603BAL1
CSB-EP320603BAL1
CSB-BP320603BAL1
CSB-MP320603BAL1
CSB-EP320603BAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zymomonas mobilis Proline--tRNA ligase (proS) CSB-YP320604ZBM
CSB-EP320604ZBM
CSB-BP320604ZBM
CSB-MP320604ZBM
CSB-EP320604ZBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus californicus Conotoxin Cl6d CSB-YP320605DVU
CSB-EP320605DVU
CSB-BP320605DVU
CSB-MP320605DVU
CSB-EP320605DVU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus quercinus Conotoxin Qc6.2 CSB-YP320606CXE
CSB-EP320606CXE
CSB-BP320606CXE
CSB-MP320606CXE
CSB-EP320606CXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vipera ammodytes ammodytes Zinc metalloproteinase ammodytagin CSB-YP320607VFO
CSB-EP320607VFO
CSB-BP320607VFO
CSB-MP320607VFO
CSB-EP320607VFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops jararacussu Thrombin-like enzyme BJ-48 CSB-YP320608BUX
CSB-EP320608BUX
CSB-BP320608BUX
CSB-MP320608BUX
CSB-EP320608BUX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant TnP I resolvase (tnpI) CSB-YP320609BRN
CSB-EP320609BRN
CSB-BP320609BRN
CSB-MP320609BRN
CSB-EP320609BRN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant C4-dicarboxylate transport transcriptional regulatory protein dctD (dctD) CSB-YP320610RKN
CSB-EP320610RKN
CSB-BP320610RKN
CSB-MP320610RKN
CSB-EP320610RKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L11 (rplK) CSB-YP320611EZA
CSB-EP320611EZA
CSB-BP320611EZA
CSB-MP320611EZA
CSB-EP320611EZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sphenodon punctatus Hemoglobin subunit beta-1 (HBB1) CSB-YP320612SJJ
CSB-EP320612SJJ
CSB-BP320612SJJ
CSB-MP320612SJJ
CSB-EP320612SJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein white (w), partial CSB-YP320613DLU1
CSB-EP320613DLU1
CSB-BP320613DLU1
CSB-MP320613DLU1
CSB-EP320613DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mojavensis Esterase-4 (Est-4) CSB-YP320614DLY
CSB-EP320614DLY
CSB-BP320614DLY
CSB-MP320614DLY
CSB-EP320614DLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Photosystem I iron-sulfur center (psaC) CSB-YP320615FKI
CSB-EP320615FKI
CSB-BP320615FKI
CSB-MP320615FKI
CSB-EP320615FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Rare lipoprotein A (rlpA) CSB-YP320616ENV
CSB-EP320616ENV
CSB-BP320616ENV
CSB-MP320616ENV
CSB-EP320616ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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