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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant UPF0135 protein SpyM3_0644 (SpyM3_0644) CSB-YP320679SMV
CSB-EP320679SMV
CSB-BP320679SMV
CSB-MP320679SMV
CSB-EP320679SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0213 protein SpyM3_1077 (SpyM3_1077) CSB-YP320680SMV
CSB-EP320680SMV
CSB-BP320680SMV
CSB-MP320680SMV
CSB-EP320680SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 UPF0297 protein SPs1796 (SPs1796) CSB-YP320681SMW
CSB-EP320681SMW
CSB-BP320681SMW
CSB-MP320681SMW
CSB-EP320681SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 UPF0348 protein SPs1630 (SPs1630) CSB-YP320682SMW
CSB-EP320682SMW
CSB-BP320682SMW
CSB-MP320682SMW
CSB-EP320682SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Undecaprenyl-diphosphatase (uppP), partial CSB-YP320683SMW
CSB-EP320683SMW
CSB-BP320683SMW
CSB-MP320683SMW
CSB-EP320683SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Uridine kinase (udk) CSB-YP320684SMW
CSB-EP320684SMW
CSB-BP320684SMW
CSB-MP320684SMW
CSB-EP320684SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine recombinase XerD-like (SpyM3_0266) CSB-YP320685SMV
CSB-EP320685SMV
CSB-BP320685SMV
CSB-MP320685SMV
CSB-EP320685SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP320686SMW
CSB-EP320686SMW
CSB-BP320686SMW
CSB-MP320686SMW
CSB-EP320686SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Major outer membrane protein P.IA (porA) CSB-YP320687NGG
CSB-BP320687NGG
CSB-MP320687NGG
CSB-EP320687NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Selaginella moellendorffii SCA7 domain-containing protein SELMODRAFT_439258 (SELMODRAFT_439258) CSB-YP320688SYF
CSB-EP320688SYF
CSB-BP320688SYF
CSB-MP320688SYF
CSB-EP320688SYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Orotate phosphoribosyltransferase (pyrE) CSB-YP320689ELW
CSB-EP320689ELW
CSB-BP320689ELW
CSB-MP320689ELW
CSB-EP320689ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative inactive G-type lectin S-receptor-like serine/threonine-protein kinase SRK (PSEUDOSRKA), partial CSB-YP320690DOA
CSB-EP320690DOA
CSB-BP320690DOA
CSB-MP320690DOA
CSB-EP320690DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At1g24800 (At1g24800) CSB-YP320691DOA
CSB-EP320691DOA
CSB-BP320691DOA
CSB-MP320691DOA
CSB-EP320691DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At1g25211 (At1g25211) CSB-YP320692DOA
CSB-EP320692DOA
CSB-BP320692DOA
CSB-MP320692DOA
CSB-EP320692DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Porin omp2b (omp2b) CSB-YP320693BMQ
CSB-EP320693BMQ
CSB-BP320693BMQ
CSB-MP320693BMQ
CSB-EP320693BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterometrus petersii Peptide Hp1090 CSB-YP320694HGR
CSB-EP320694HGR
CSB-BP320694HGR
CSB-MP320694HGR
CSB-EP320694HGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterometrus petersii Potassium channel toxin kappa-KTx 3.1 CSB-YP320695HGR
CSB-EP320695HGR
CSB-BP320695HGR
CSB-MP320695HGR
CSB-EP320695HGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heterometrus petersii Potassium channel toxin kappa-KTx 3.4 CSB-YP320696HGR
CSB-EP320696HGR
CSB-BP320696HGR
CSB-MP320696HGR
CSB-EP320696HGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum HNTX-03141007 CSB-YP320697HUK
CSB-EP320697HUK
CSB-BP320697HUK
CSB-MP320697HUK
CSB-EP320697HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum HNTX-0109076 CSB-YP320698HUK
CSB-EP320698HUK
CSB-BP320698HUK
CSB-MP320698HUK
CSB-EP320698HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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