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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Triticum aestivum Alpha-amylase/trypsin inhibitor CM1 CSB-YP322320TQN
CSB-EP322320TQN
CSB-BP322320TQN
CSB-MP322320TQN
CSB-EP322320TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Volvox carteri Histone H2B.3 CSB-YP322321VDT
CSB-EP322321VDT
CSB-BP322321VDT
CSB-MP322321VDT
CSB-EP322321VDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovine maedi visna related virus Gag polyprotein (gag) CSB-YP322322OCW
CSB-EP322322OCW
CSB-BP322322OCW
CSB-MP322322OCW
CSB-EP322322OCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Serine/threonine-protein kinase 1 (VPK1) CSB-YP322323VAI
CSB-EP322323VAI
CSB-BP322323VAI
CSB-MP322323VAI
CSB-EP322323VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia 2,2-dialkylglycine decarboxylase (dgdA) CSB-YP322324BXR
CSB-EP322324BXR
CSB-BP322324BXR
CSB-MP322324BXR
CSB-EP322324BXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS630 uncharacterized 39 kDa protein CSB-YP322325SGH
CSB-EP322325SGH
CSB-BP322325SGH
CSB-MP322325SGH
CSB-EP322325SGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoanaerobacter thermohydrosulfuricus Amylopullulanase (apu), partial CSB-YP322326TCY
CSB-EP322326TCY
CSB-BP322326TCY
CSB-MP322326TCY
CSB-EP322326TCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyltransferase (ttr) CSB-YP322327PWF
CSB-EP322327PWF
CSB-BP322327PWF
CSB-MP322327PWF
CSB-EP322327PWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP322328IGU
CSB-EP322328IGU
CSB-BP322328IGU
CSB-MP322328IGU
CSB-EP322328IGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP322329IDH
CSB-EP322329IDH
CSB-BP322329IDH
CSB-MP322329IDH
CSB-EP322329IDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP322330IDZ
CSB-EP322330IDZ
CSB-BP322330IDZ
CSB-MP322330IDZ
CSB-EP322330IDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanelle 30S ribosomal protein S10 (rps10) CSB-YP322331DZX
CSB-EP322331DZX
CSB-BP322331DZX
CSB-MP322331DZX
CSB-EP322331DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Zinc finger protein 30 (ZNF30) CSB-YP322332HU
CSB-EP322332HU
CSB-BP322332HU
CSB-MP322332HU
CSB-EP322332HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Hydroxyneurosporene dehydrogenase (crtC) CSB-YP322333RLD
CSB-EP322333RLD
CSB-BP322333RLD
CSB-MP322333RLD
CSB-EP322333RLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gossypium hirsutum Isocitrate lyase, partial CSB-YP322334GHB
CSB-EP322334GHB
CSB-BP322334GHB
CSB-MP322334GHB
CSB-EP322334GHB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Urease accessory protein UreE (ureE) CSB-YP322335EYZ
CSB-EP322335EYZ
CSB-BP322335EYZ
CSB-MP322335EYZ
CSB-EP322335EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Kinesin-like protein KAR3 (KAR3), partial CSB-YP322336SVG
CSB-EP322336SVG
CSB-BP322336SVG
CSB-MP322336SVG
CSB-EP322336SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Heterogeneous nuclear ribonucleoproteins A1 homolog (hnrnpa1) CSB-YP322337XBE
CSB-EP322337XBE
CSB-BP322337XBE
CSB-MP322337XBE
CSB-EP322337XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein UL65 (UL65) CSB-YP322338HWV
CSB-EP322338HWV
CSB-BP322338HWV
CSB-MP322338HWV
CSB-EP322338HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0042 nucleotide-binding protein in ptsN-ptsO intergenic region (yhbJ) CSB-YP322339KBE
CSB-EP322339KBE
CSB-BP322339KBE
CSB-MP322339KBE
CSB-EP322339KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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