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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Probable 40S ribosomal protein S9 CSB-YP321570TQV
CSB-EP321570TQV
CSB-BP321570TQV
CSB-MP321570TQV
CSB-EP321570TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pesticidal crystal protein cry3Ba (cry3Ba), partial CSB-YP321571BDH
CSB-EP321571BDH
CSB-BP321571BDH
CSB-MP321571BDH
CSB-EP321571BDH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein psiA (psiA) CSB-YP321572ENL
CSB-EP321572ENL
CSB-BP321572ENL
CSB-MP321572ENL
CSB-EP321572ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat T-cell ecto-ADP-ribosyltransferase 1 (Art2a) CSB-YP321573RA
CSB-EP321573RA
CSB-BP321573RA
CSB-MP321573RA
CSB-EP321573RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yfaA (yfaA) CSB-YP321574ENV
CSB-EP321574ENV
CSB-BP321574ENV
CSB-MP321574ENV
CSB-EP321574ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein trbI (trbI), partial CSB-YP321575ENV1
CSB-EP321575ENV1
CSB-BP321575ENV1
CSB-MP321575ENV1
CSB-EP321575ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Copy number protein (cop) CSB-YP321576CMB
CSB-EP321576CMB
CSB-BP321576CMB
CSB-MP321576CMB
CSB-EP321576CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication initiation protein (repZ) CSB-YP321577ENL
CSB-EP321577ENL
CSB-BP321577ENL
CSB-MP321577ENL
CSB-EP321577ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T6 Deoxycytidylate 5-hydroxymethyltransferase (42) CSB-YP321578EEA
CSB-EP321578EEA
CSB-BP321578EEA
CSB-MP321578EEA
CSB-EP321578EEA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 5 CSB-YP321579RGP
CSB-EP321579RGP
CSB-BP321579RGP
CSB-MP321579RGP
CSB-EP321579RGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Gag-Pol polyprotein (gag-pol), partial CSB-YP321580HLX
CSB-EP321580HLX
CSB-BP321580HLX
CSB-MP321580HLX
CSB-EP321580HLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP321581IHV
CSB-EP321581IHV
CSB-BP321581IHV
CSB-MP321581IHV
CSB-EP321581IHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Nef (nef) CSB-YP321582HLU
CSB-EP321582HLU
CSB-BP321582HLU
CSB-MP321582HLU
CSB-EP321582HLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Protein Tat (tat) CSB-YP321583HLU
CSB-EP321583HLU
CSB-BP321583HLU
CSB-MP321583HLU
CSB-EP321583HLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica 3-isopropylmalate dehydrogenase (LEU2) CSB-YP321584YAP
CSB-EP321584YAP
CSB-BP321584YAP
CSB-MP321584YAP
CSB-EP321584YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tacaribe virus Nucleoprotein (N), partial CSB-YP321585TAC
CSB-EP321585TAC
CSB-BP321585TAC
CSB-MP321585TAC
CSB-EP321585TAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein psiA (psiA) CSB-YP321586ENV
CSB-EP321586ENV
CSB-BP321586ENV
CSB-MP321586ENV
CSB-EP321586ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Defective chorion-1 protein, FC106 isoform (dec-1), partial CSB-YP321588DLU
CSB-EP321588DLU
CSB-BP321588DLU
CSB-MP321588DLU
CSB-EP321588DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridioides difficile Toxin B (toxB), partial CSB-YP321589DUN
CSB-EP321589DUN
CSB-BP321589DUN
CSB-MP321589DUN
CSB-EP321589DUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uptake hydrogenase small subunit (hupA) CSB-YP321590DPU
CSB-EP321590DPU
CSB-BP321590DPU
CSB-MP321590DPU
CSB-EP321590DPU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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