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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rat L-gulonolactone oxidase (Gulo), partial CSB-YP320800RA1
CSB-EP320800RA1
CSB-BP320800RA1
CSB-MP320800RA1
CSB-EP320800RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protophormia terraenovae Phormicin CSB-YP320801PST
CSB-EP320801PST
CSB-BP320801PST
CSB-MP320801PST
CSB-EP320801PST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic (RCA) CSB-YP320802DOA
CSB-EP320802DOA
CSB-BP320802DOA
CSB-MP320802DOA
CSB-EP320802DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Alpha-L-fucosidase (alfA) CSB-YP320803DKK
CSB-EP320803DKK
CSB-BP320803DKK
CSB-MP320803DKK
CSB-EP320803DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli sn-glycerol-3-phosphate transport system permease protein ugpA (ugpA), partial CSB-YP320804ENV1
CSB-EP320804ENV1
CSB-BP320804ENV1
CSB-MP320804ENV1
CSB-EP320804ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycerophosphoryl diester phosphodiesterase (ugpQ) CSB-YP320805ENV
CSB-EP320805ENV
CSB-BP320805ENV
CSB-MP320805ENV
CSB-EP320805ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea batatas Sporamin A (GSPO-A1) CSB-YP320806INB
CSB-EP320806INB
CSB-BP320806INB
CSB-MP320806INB
CSB-EP320806INB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Baseplate structural protein Gp9 (9) CSB-YP320807EDZ
CSB-EP320807EDZ
CSB-BP320807EDZ
CSB-MP320807EDZ
CSB-EP320807EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Baseplate structural protein Gp11 (11) CSB-YP320808EDZ
CSB-EP320808EDZ
CSB-BP320808EDZ
CSB-MP320808EDZ
CSB-EP320808EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Hut operon positive regulatory protein (hutP) CSB-YP320809BRJ
CSB-EP320809BRJ
CSB-BP320809BRJ
CSB-MP320809BRJ
CSB-EP320809BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Sensor protein fixL (fixL), partial CSB-YP320810RKU1
CSB-EP320810RKU1
CSB-BP320810RKU1
CSB-MP320810RKU1
CSB-EP320810RKU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein MAK16 (MAK16) CSB-YP320811SVG
CSB-EP320811SVG
CSB-BP320811SVG
CSB-MP320811SVG
CSB-EP320811SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 50S ribosomal protein L22P (rpl22p) CSB-YP320812HTJ
CSB-EP320812HTJ
CSB-BP320812HTJ
CSB-MP320812HTJ
CSB-EP320812HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ricinus communis Non-specific lipid-transfer protein A CSB-YP320813RMM
CSB-EP320813RMM
CSB-BP320813RMM
CSB-MP320813RMM
CSB-EP320813RMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Absidia glauca Actin-1 (ACT1), partial CSB-YP320814AAD
CSB-BP320814AAD
CSB-MP320814AAD
CSB-EP320814AAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actin-4 (act-4) CSB-YP320815CXY
CSB-EP320815CXY
CSB-BP320815CXY
CSB-MP320815CXY
CSB-EP320815CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus franciscanus Actin-15A CSB-YP320816SQT
CSB-EP320816SQT
CSB-BP320816SQT
CSB-MP320816SQT
CSB-EP320816SQT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pleurodeles waltl Actin, alpha skeletal muscle CSB-YP320817EWZ
CSB-EP320817EWZ
CSB-BP320817EWZ
CSB-MP320817EWZ
CSB-EP320817EWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Didelphis marsupialis virginiana Hemoglobin subunit epsilon-M (HBE1) CSB-YP320818DKL
CSB-EP320818DKL
CSB-BP320818DKL
CSB-MP320818DKL
CSB-EP320818DKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Granzyme D (Gzmd) CSB-YP320819MO
CSB-EP320819MO
CSB-BP320819MO
CSB-MP320819MO
CSB-EP320819MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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