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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster Protein extra-macrochaetae (emc) CSB-YP322441DLU
CSB-EP322441DLU
CSB-BP322441DLU
CSB-MP322441DLU
CSB-EP322441DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein patched (ptc), partial CSB-YP322442DLU
CSB-EP322442DLU
CSB-BP322442DLU
CSB-MP322442DLU
CSB-EP322442DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus textile Delta-conotoxin TxVIA CSB-YP322443DWJ
CSB-EP322443DWJ
CSB-BP322443DWJ
CSB-MP322443DWJ
CSB-EP322443DWJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Intermediate filament protein ON3, partial CSB-YP322444DQG
CSB-EP322444DQG
CSB-BP322444DQG
CSB-MP322444DQG
CSB-EP322444DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig heavy chain V region 5-84 CSB-YP322445MO
CSB-EP322445MO
CSB-BP322445MO
CSB-MP322445MO
CSB-EP322445MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig heavy chain V region M315 CSB-YP322446MO
CSB-EP322446MO
CSB-BP322446MO
CSB-MP322446MO
CSB-EP322446MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gallid herpesvirus 2 Envelope glycoprotein B (gB), partial CSB-YP322447GAQ
CSB-EP322447GAQ
CSB-BP322447GAQ
CSB-MP322447GAQ
CSB-EP322447GAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Phycobilisome 27.9 kDa linker polypeptide, phycoerythrin-associated, rod (cpeD) CSB-YP322448FCY
CSB-EP322448FCY
CSB-BP322448FCY
CSB-MP322448FCY
CSB-EP322448FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isopenicillin N epimerase (cefD) CSB-YP322449FOA
CSB-EP322449FOA
CSB-BP322449FOA
CSB-MP322449FOA
CSB-EP322449FOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein MGF 110-4L (BA71V-011, BA71V-012) CSB-YP322450AEJ
CSB-EP322450AEJ
CSB-BP322450AEJ
CSB-MP322450AEJ
CSB-EP322450AEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus B Outer capsid glycoprotein VP7, partial CSB-YP322451RHE
CSB-EP322451RHE
CSB-BP322451RHE
CSB-MP322451RHE
CSB-EP322451RHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mustela vison Serum amyloid A-1 protein (SAA1) CSB-YP322452MVE
CSB-EP322452MVE
CSB-BP322452MVE
CSB-MP322452MVE
CSB-EP322452MVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens T-DNA border endonuclease virD1 (virD1) CSB-YP322453AYS
CSB-EP322453AYS
CSB-BP322453AYS
CSB-MP322453AYS
CSB-EP322453AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Elongation factor 1-alpha (eef1a1) CSB-YP322454DKK
CSB-EP322454DKK
CSB-BP322454DKK
CSB-MP322454DKK
CSB-EP322454DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Ribosomal protein S6 kinase 2 alpha (RPS6KA), partial CSB-YP322456CH
CSB-EP322456CH
CSB-BP322456CH
CSB-MP322456CH
CSB-EP322456CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max 50S ribosomal protein L2-A, chloroplastic (rpl2-A) CSB-YP322457GGV
CSB-EP322457GGV
CSB-BP322457GGV
CSB-MP322457GGV
CSB-EP322457GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maclura pomifera Agglutinin alpha chain CSB-YP322458MAD
CSB-EP322458MAD
CSB-BP322458MAD
CSB-MP322458MAD
CSB-EP322458MAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage HK022 26 kDa repressor protein (CI-HTT) CSB-YP322459ECN
CSB-EP322459ECN
CSB-BP322459ECN
CSB-MP322459ECN
CSB-EP322459ECN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dactylis glomerata Prolamin alpha-3 CSB-YP322460DAC
CSB-EP322460DAC
CSB-BP322460DAC
CSB-MP322460DAC
CSB-EP322460DAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Vitellogenin-A2, partial CSB-YP322461XBE
CSB-EP322461XBE
CSB-BP322461XBE
CSB-MP322461XBE
CSB-EP322461XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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