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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rhyparobia maderae Leucokinin-7 CSB-YP321754REJ
CSB-EP321754REJ
CSB-BP321754REJ
CSB-MP321754REJ
CSB-EP321754REJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phlebia radiata Ligninase-3 CSB-YP321755PHO
CSB-BP321755PHO
CSB-MP321755PHO
CSB-EP321755PHO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Varecia variegata Hemoglobin subunit alpha-1 CSB-YP321756VAN
CSB-EP321756VAN
CSB-BP321756VAN
CSB-MP321756VAN
CSB-EP321756VAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Hypoxanthine-guanine-xanthine phosphoribosyltransferase (LACZ) CSB-YP321757PLW
CSB-EP321757PLW
CSB-BP321757PLW
CSB-MP321757PLW
CSB-EP321757PLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia solanacearum Polygalacturonase (pglA), partial CSB-YP321758RJJ
CSB-EP321758RJJ
CSB-BP321758RJJ
CSB-MP321758RJJ
CSB-EP321758RJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmid recombination enzyme (preA) CSB-YP321759LMR
CSB-EP321759LMR
CSB-BP321759LMR
CSB-MP321759LMR
CSB-EP321759LMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Prephenate dehydrogenase [NADP (+)] CSB-YP321760SVG
CSB-EP321760SVG
CSB-BP321760SVG
CSB-MP321760SVG
CSB-EP321760SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Protein PYR1-3 (pyr1-3), partial CSB-YP321761DKK
CSB-EP321761DKK
CSB-BP321761DKK
CSB-MP321761DKK
CSB-EP321761DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloramphenicol acetyltransferase (cat) CSB-YP321762SNG
CSB-EP321762SNG
CSB-BP321762SNG
CSB-MP321762SNG
CSB-EP321762SNG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Peptidyl-prolyl cis-trans isomerase fkr-2 (fkr-2) CSB-YP321763NHA
CSB-EP321763NHA
CSB-BP321763NHA
CSB-MP321763NHA
CSB-EP321763NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Photosystem II CP47 chlorophyll apoprotein (psbB), partial CSB-YP321765NHR1
CSB-EP321765NHR1
CSB-BP321765NHR1
CSB-MP321765NHR1
CSB-EP321765NHR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia amylovora Colanic acid capsular biosynthesis activation protein A (rcsA) CSB-YP321766EMS
CSB-EP321766EMS
CSB-BP321766EMS
CSB-MP321766EMS
CSB-EP321766EMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Zinc/cadmium resistance protein (ZRC1), partial CSB-YP321767SVG1
CSB-EP321767SVG1
CSB-BP321767SVG1
CSB-MP321767SVG1
CSB-EP321767SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Photosystem I reaction center subunit II (psaD) CSB-YP321768EWE
CSB-EP321768EWE
CSB-BP321768EWE
CSB-MP321768EWE
CSB-EP321768EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus Replication protein 1a (ORF1a), partial CSB-YP321769CUA
CSB-EP321769CUA
CSB-BP321769CUA
CSB-MP321769CUA
CSB-EP321769CUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA replicase polyprotein, partial CSB-YP321770EAV
CSB-EP321770EAV
CSB-BP321770EAV
CSB-MP321770EAV
CSB-EP321770EAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 66 kDa protein, partial CSB-YP321771OBP
CSB-EP321771OBP
CSB-BP321771OBP
CSB-MP321771OBP
CSB-EP321771OBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Heat shock 90 kDa protein homolog CSB-YP321772PMD
CSB-EP321772PMD
CSB-BP321772PMD
CSB-MP321772PMD
CSB-EP321772PMD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Lipopolysaccharide export system ATP-binding protein LptB (lptB) CSB-YP321773FFZ
CSB-EP321773FFZ
CSB-BP321773FFZ
CSB-MP321773FFZ
CSB-EP321773FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis PTS system glucose-specific EIICBA component (ptsG), partial CSB-YP321774BRJ
CSB-EP321774BRJ
CSB-BP321774BRJ
CSB-MP321774BRJ
CSB-EP321774BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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