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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Human cytomegalovirus Tegument protein UL23 (UL23) CSB-YP322891HWV
CSB-EP322891HWV
CSB-BP322891HWV
CSB-MP322891HWV
CSB-EP322891HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Culex pipiens Esterase B1 (B1), partial CSB-YP322892CUK
CSB-EP322892CUK
CSB-BP322892CUK
CSB-MP322892CUK
CSB-EP322892CUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gap junction alpha-2 protein (gja2), partial CSB-YP322893XBE1
CSB-EP322893XBE1
CSB-BP322893XBE1
CSB-MP322893XBE1
CSB-EP322893XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus F serotype 41 Fiber protein 2 CSB-YP322894HIS
CSB-EP322894HIS
CSB-BP322894HIS
CSB-MP322894HIS
CSB-EP322894HIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitogen-activated protein kinase FUS3 (FUS3) CSB-YP322895SVG
CSB-EP322895SVG
CSB-BP322895SVG
CSB-MP322895SVG
CSB-EP322895SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovine maedi visna related virus Pol polyprotein (pol) CSB-YP322896OCW
CSB-EP322896OCW
CSB-BP322896OCW
CSB-MP322896OCW
CSB-EP322896OCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein elav (elav) CSB-YP322897DLU
CSB-EP322897DLU
CSB-BP322897DLU
CSB-MP322897DLU
CSB-EP322897DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizophyllum commune Fruiting body protein SC3 (SC3) CSB-YP322898SXR
CSB-EP322898SXR
CSB-BP322898SXR
CSB-MP322898SXR
CSB-EP322898SXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequence element IS629 CSB-YP322899SGH
CSB-EP322899SGH
CSB-BP322899SGH
CSB-MP322899SGH
CSB-EP322899SGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATPase-stabilizing factor 15 kDa protein (STF2) CSB-YP322900SVG
CSB-EP322900SVG
CSB-BP322900SVG
CSB-MP322900SVG
CSB-EP322900SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP322901IFO
CSB-EP322901IFO
CSB-BP322901IFO
CSB-MP322901IFO
CSB-EP322901IFO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Hemagglutinin-esterase-fusion glycoprotein (HE), partial CSB-YP322902IKL
CSB-EP322902IKL
CSB-BP322902IKL
CSB-MP322902IKL
CSB-EP322902IKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rubredoxin-NAD (+) reductase CSB-YP322903PUQ
CSB-EP322903PUQ
CSB-BP322903PUQ
CSB-MP322903PUQ
CSB-EP322903PUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Phytoene synthase (crtB) CSB-YP322904RLD
CSB-EP322904RLD
CSB-BP322904RLD
CSB-MP322904RLD
CSB-EP322904RLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum 30S ribosomal protein S17, chloroplastic (RPS17) CSB-YP322905EWE
CSB-EP322905EWE
CSB-BP322905EWE
CSB-MP322905EWE
CSB-EP322905EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype A2 subtype adw2 External core antigen (C) CSB-YP322906HDO
CSB-EP322906HDO
CSB-BP322906HDO
CSB-MP322906HDO
CSB-EP322906HDO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD) CSB-YP322907ENV
CSB-EP322907ENV
CSB-BP322907ENV
CSB-MP322907ENV
CSB-EP322907ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Kinesin-like protein bimC (bimC), partial CSB-YP322908EKF
CSB-EP322908EKF
CSB-BP322908EKF
CSB-MP322908EKF
CSB-EP322908EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helobdella triserialis Homeobox protein HTR-A2 CSB-YP322909HDD
CSB-EP322909HDD
CSB-BP322909HDD
CSB-MP322909HDD
CSB-EP322909HDD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Early glycoprotein GP48 (UL4) CSB-YP322910HWV
CSB-EP322910HWV
CSB-BP322910HWV
CSB-MP322910HWV
CSB-EP322910HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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