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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Macaca assamensis Hemoglobin subunit alpha-1/2/3 CSB-YP322022MMV
CSB-EP322022MMV
CSB-BP322022MMV
CSB-MP322022MMV
CSB-EP322022MMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 40S ribosomal protein S26E (rps-26) CSB-YP322023NHA
CSB-EP322023NHA
CSB-BP322023NHA
CSB-MP322023NHA
CSB-EP322023NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus 6-phosphofructokinase isozyme 1 (pfkA) CSB-YP322024TNT
CSB-EP322024TNT
CSB-BP322024TNT
CSB-MP322024TNT
CSB-EP322024TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Neurogenic locus notch protein homolog (notch1), partial CSB-YP322025XBE
CSB-EP322025XBE
CSB-BP322025XBE
CSB-MP322025XBE
CSB-EP322025XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemicentrotus pulcherrimus 15 kDa calcium-binding protein CSB-YP322026HVL
CSB-EP322026HVL
CSB-BP322026HVL
CSB-MP322026HVL
CSB-EP322026HVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micrurus nigrocinctus Phospholipase A2 isozyme 1 CSB-YP322027MGO
CSB-EP322027MGO
CSB-BP322027MGO
CSB-MP322027MGO
CSB-EP322027MGO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDH2) CSB-YP322028SVG
CSB-EP322028SVG
CSB-BP322028SVG
CSB-MP322028SVG
CSB-EP322028SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Chemotaxis protein CheA (cheA), partial CSB-YP322029EKO
CSB-EP322029EKO
CSB-BP322029EKO
CSB-MP322029EKO
CSB-EP322029EKO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Chemotaxis protein CheW (cheW) CSB-YP322030EKO
CSB-EP322030EKO
CSB-BP322030EKO
CSB-MP322030EKO
CSB-EP322030EKO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes Methyl-accepting chemotaxis serine transducer (tse), partial CSB-YP322031EKO1
CSB-EP322031EKO1
CSB-BP322031EKO1
CSB-MP322031EKO1
CSB-EP322031EKO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Malate synthase 2, glyoxysomal (DAL7), partial CSB-YP322032SVG
CSB-EP322032SVG
CSB-BP322032SVG
CSB-MP322032SVG
CSB-EP322032SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Fibroin light chain (FIBL) CSB-YP322033BTT
CSB-EP322033BTT
CSB-BP322033BTT
CSB-MP322033BTT
CSB-EP322033BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum Endoglucanase 2 CSB-YP322034SDU
CSB-EP322034SDU
CSB-BP322034SDU
CSB-MP322034SDU
CSB-EP322034SDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Major surface-labeled trophozoite antigen 417 (TSA 417), partial CSB-YP322035GFW
CSB-EP322035GFW
CSB-BP322035GFW
CSB-MP322035GFW
CSB-EP322035GFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Periplasmic [NiFe] hydrogenase small subunit (hydA) CSB-YP322036DJR
CSB-EP322036DJR
CSB-BP322036DJR
CSB-MP322036DJR
CSB-EP322036DJR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Phosphinothricin N-acetyltransferase (bar) CSB-YP322038FOB
CSB-EP322038FOB
CSB-BP322038FOB
CSB-MP322038FOB
CSB-EP322038FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sensor protein KdpD (kdpD), partial CSB-YP322039ENV
CSB-EP322039ENV
CSB-BP322039ENV
CSB-MP322039ENV
CSB-EP322039ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Trifunctional purine biosynthetic protein adenosine-3 (GART), partial CSB-YP322040CH
CSB-EP322040CH
CSB-BP322040CH
CSB-MP322040CH
CSB-EP322040CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variable large protein 7 (vlp7) CSB-YP322041BIP
CSB-EP322041BIP
CSB-BP322041BIP
CSB-MP322041BIP
CSB-EP322041BIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small, acid-soluble spore protein C1 (sspC1) CSB-YP322042CMB
CSB-EP322042CMB
CSB-BP322042CMB
CSB-MP322042CMB
CSB-EP322042CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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