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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Haloferax volcanii Tryptophan synthase alpha chain (trpA) CSB-YP322428HTU
CSB-EP322428HTU
CSB-BP322428HTU
CSB-MP322428HTU
CSB-EP322428HTU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloferax volcanii Indole-3-glycerol phosphate synthase (trpC) CSB-YP322429HTU
CSB-EP322429HTU
CSB-BP322429HTU
CSB-MP322429HTU
CSB-EP322429HTU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sarcophaga peregrina Sapecin CSB-YP322430SCK
CSB-EP322430SCK
CSB-BP322430SCK
CSB-MP322430SCK
CSB-EP322430SCK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Endoglucanase CSB-YP322431CFR
CSB-EP322431CFR
CSB-BP322431CFR
CSB-MP322431CFR
CSB-EP322431CFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiriine herpesvirus 2 Transforming protein STP (1), partial CSB-YP322432SAX1
CSB-EP322432SAX1
CSB-BP322432SAX1
CSB-MP322432SAX1
CSB-EP322432SAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dengue virus type 2 Genome polyprotein, partial CSB-YP322433DCH
CSB-EP322433DCH
CSB-BP322433DCH
CSB-MP322433DCH
CSB-EP322433DCH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Serine proteinase inhibitor 3 (SPI-3) CSB-YP322434VAI
CSB-EP322434VAI
CSB-BP322434VAI
CSB-MP322434VAI
CSB-EP322434VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Nitrogen fixation protein fixH (fixH), partial CSB-YP322435RKU1
CSB-EP322435RKU1
CSB-BP322435RKU1
CSB-MP322435RKU1
CSB-EP322435RKU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sporulation-specific protein SPO7 (SPO7), partial CSB-YP322436SVG
CSB-EP322436SVG
CSB-BP322436SVG
CSB-MP322436SVG
CSB-EP322436SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Murine coronavirus Protein I (N) CSB-YP322437MJU
CSB-EP322437MJU
CSB-BP322437MJU
CSB-MP322437MJU
CSB-EP322437MJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HLA class I histocompatibility antigen, B-37 alpha chain (HLA-B), partial CSB-YP322438HU
CSB-EP322438HU
CSB-BP322438HU
CSB-MP322438HU
CSB-EP322438HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Periplasmic protein trbC (trbC) CSB-YP322439ENV
CSB-EP322439ENV
CSB-BP322439ENV
CSB-MP322439ENV
CSB-EP322439ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trypsin-resistant surface T6 protein (tee6) CSB-YP322440SMZ
CSB-EP322440SMZ
CSB-BP322440SMZ
CSB-MP322440SMZ
CSB-EP322440SMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein extra-macrochaetae (emc) CSB-YP322441DLU
CSB-EP322441DLU
CSB-BP322441DLU
CSB-MP322441DLU
CSB-EP322441DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein patched (ptc), partial CSB-YP322442DLU
CSB-EP322442DLU
CSB-BP322442DLU
CSB-MP322442DLU
CSB-EP322442DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus textile Delta-conotoxin TxVIA CSB-YP322443DWJ
CSB-EP322443DWJ
CSB-BP322443DWJ
CSB-MP322443DWJ
CSB-EP322443DWJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Intermediate filament protein ON3, partial CSB-YP322444DQG
CSB-EP322444DQG
CSB-BP322444DQG
CSB-MP322444DQG
CSB-EP322444DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig heavy chain V region 5-84 CSB-YP322445MO
CSB-EP322445MO
CSB-BP322445MO
CSB-MP322445MO
CSB-EP322445MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig heavy chain V region M315 CSB-YP322446MO
CSB-EP322446MO
CSB-BP322446MO
CSB-MP322446MO
CSB-EP322446MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gallid herpesvirus 2 Envelope glycoprotein B (gB), partial CSB-YP322447GAQ
CSB-EP322447GAQ
CSB-BP322447GAQ
CSB-MP322447GAQ
CSB-EP322447GAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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