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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Probable nitrate reductase molybdenum cofactor assembly chaperone NarW (narW) CSB-YP322522ENV
CSB-EP322522ENV
CSB-BP322522ENV
CSB-MP322522ENV
CSB-EP322522ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni 23 kDa integral membrane protein, partial CSB-YP322523SXQ1
CSB-EP322523SXQ1
CSB-BP322523SXQ1
CSB-MP322523SXQ1
CSB-EP322523SXQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia sp. Extracellular globin-E7 CSB-YP322524AOF
CSB-EP322524AOF
CSB-BP322524AOF
CSB-MP322524AOF
CSB-EP322524AOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein snail homolog Sna (snai1) CSB-YP322525XBE
CSB-EP322525XBE
CSB-BP322525XBE
CSB-MP322525XBE
CSB-EP322525XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Capsule polysaccharide export inner-membrane protein BexB (bexB), partial CSB-YP322526HSZ1
CSB-EP322526HSZ1
CSB-BP322526HSZ1
CSB-MP322526HSZ1
CSB-EP322526HSZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ascaris suum Major pepsin inhibitor 3 (PI-3) CSB-YP322527DOS
CSB-BP322527DOS
CSB-MP322527DOS
CSB-EP322527DOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pesticidal crystal protein cry1Da (cry1Da), partial CSB-YP322528BCU
CSB-EP322528BCU
CSB-BP322528BCU
CSB-MP322528BCU
CSB-EP322528BCU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii 10 kDa chaperonin (groS) CSB-YP322529DXP
CSB-EP322529DXP
CSB-BP322529DXP
CSB-MP322529DXP
CSB-EP322529DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nodulation protein S (nodS) CSB-YP322530RKX
CSB-EP322530RKX
CSB-BP322530RKX
CSB-MP322530RKX
CSB-EP322530RKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gluconacetobacter xylinus Cellulose synthase catalytic subunit [UDP-forming] (bcsA), partial CSB-YP322531GGT
CSB-EP322531GGT
CSB-BP322531GGT
CSB-MP322531GGT
CSB-EP322531GGT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 25 kDa ookinete surface antigen CSB-YP322532PMP
CSB-EP322532PMP
CSB-BP322532PMP
CSB-MP322532PMP
CSB-EP322532PMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guillardia theta 30S ribosomal protein S10, chloroplastic (rps10) CSB-YP322533GHG
CSB-EP322533GHG
CSB-BP322533GHG
CSB-MP322533GHG
CSB-EP322533GHG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Persea americana 1-aminocyclopropane-1-carboxylate oxidase (ACO) CSB-YP322534PGI
CSB-EP322534PGI
CSB-BP322534PGI
CSB-MP322534PGI
CSB-EP322534PGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma japonicum Eggshell protein 1 (ESG-1) CSB-YP322535SXP
CSB-EP322535SXP
CSB-BP322535SXP
CSB-MP322535SXP
CSB-EP322535SXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum 34 kDa membrane antigen (tpd) CSB-YP322536TPR
CSB-BP322536TPR
CSB-MP322536TPR
CSB-EP322536TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Major extracellular endoglucanase (engXCA) CSB-YP322537XAY
CSB-EP322537XAY
CSB-BP322537XAY
CSB-MP322537XAY
CSB-EP322537XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Coenzyme F420 hydrogenase subunit alpha (frhA) CSB-YP322538MSR
CSB-EP322538MSR
CSB-BP322538MSR
CSB-MP322538MSR
CSB-EP322538MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Nef (nef) CSB-YP322539SHD
CSB-EP322539SHD
CSB-BP322539SHD
CSB-MP322539SHD
CSB-EP322539SHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovine maedi visna related virus Putative uncharacterized protein W CSB-YP322540OCW
CSB-EP322540OCW
CSB-BP322540OCW
CSB-MP322540OCW
CSB-EP322540OCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Major outer membrane porin, serovar L1 (ompA) CSB-YP322541DSA
CSB-EP322541DSA
CSB-BP322541DSA
CSB-MP322541DSA
CSB-EP322541DSA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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