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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF29.1 CSB-YP322462XBE
CSB-EP322462XBE
CSB-BP322462XBE
CSB-MP322462XBE
CSB-EP322462XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF49.1 CSB-YP322463XBE
CSB-EP322463XBE
CSB-BP322463XBE
CSB-MP322463XBE
CSB-EP322463XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF57.1 CSB-YP322464XBE
CSB-EP322464XBE
CSB-BP322464XBE
CSB-MP322464XBE
CSB-EP322464XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gastrula zinc finger protein XlCGF9.1 CSB-YP322465XBE
CSB-EP322465XBE
CSB-BP322465XBE
CSB-MP322465XBE
CSB-EP322465XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Oocyte zinc finger protein XlCOF29, partial CSB-YP322466XBE
CSB-EP322466XBE
CSB-BP322466XBE
CSB-MP322466XBE
CSB-EP322466XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Oocyte zinc finger protein XlCOF8.4, partial CSB-YP322467XBE
CSB-EP322467XBE
CSB-BP322467XBE
CSB-MP322467XBE
CSB-EP322467XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligopeptide transport ATP-binding protein AmiF (amiF) CSB-YP322468FMW
CSB-EP322468FMW
CSB-BP322468FMW
CSB-MP322468FMW
CSB-EP322468FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Large tail fiber protein p34 (34), partial CSB-YP322469EDZ
CSB-EP322469EDZ
CSB-BP322469EDZ
CSB-MP322469EDZ
CSB-EP322469EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tellurium resistance protein terC (terC), partial CSB-YP322470AGP
CSB-EP322470AGP
CSB-BP322470AGP
CSB-MP322470AGP
CSB-EP322470AGP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligopeptide-binding protein AmiA (amiA), partial CSB-YP322471FMW
CSB-EP322471FMW
CSB-BP322471FMW
CSB-MP322471FMW
CSB-EP322471FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Nef (nef) CSB-YP322472HLJ
CSB-EP322472HLJ
CSB-BP322472HLJ
CSB-MP322472HLJ
CSB-EP322472HLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype D Protein Vpu (vpu), partial CSB-YP322473HLJ
CSB-EP322473HLJ
CSB-BP322473HLJ
CSB-MP322473HLJ
CSB-EP322473HLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Maltose regulon regulatory protein malI (malI) CSB-YP322474ENV
CSB-EP322474ENV
CSB-BP322474ENV
CSB-MP322474ENV
CSB-EP322474ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyridium cruentum Ferredoxin CSB-YP322475PQN
CSB-EP322475PQN
CSB-BP322475PQN
CSB-MP322475PQN
CSB-EP322475PQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NH (3)-dependent NAD (+) synthetase CSB-YP322476ENV
CSB-EP322476ENV
CSB-BP322476ENV
CSB-MP322476ENV
CSB-EP322476ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Guanine nucleotide-binding protein subunit gamma (STE18) CSB-YP322477SVG
CSB-EP322477SVG
CSB-BP322477SVG
CSB-MP322477SVG
CSB-EP322477SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP322478IEY
CSB-EP322478IEY
CSB-BP322478IEY
CSB-MP322478IEY
CSB-EP322478IEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP322479IBY
CSB-EP322479IBY
CSB-BP322479IBY
CSB-MP322479IBY
CSB-EP322479IBY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Alginate production protein AlgE (algE) CSB-YP322480EZX
CSB-EP322480EZX
CSB-BP322480EZX
CSB-MP322480EZX
CSB-EP322480EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat D (1A) dopamine receptor (Drd1), partial CSB-YP322481RA1
CSB-EP322481RA1
CSB-BP322481RA1
CSB-MP322481RA1
CSB-EP322481RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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