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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacteria phage T3 Uncharacterized gene 1.5 protein (1.5) CSB-YP322693EDY
CSB-EP322693EDY
CSB-BP322693EDY
CSB-MP322693EDY
CSB-EP322693EDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Alpha-agglutinin (SAG1), partial CSB-YP322694SVG
CSB-EP322694SVG
CSB-BP322694SVG
CSB-MP322694SVG
CSB-EP322694SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vultur gryphus Ovomucoid CSB-YP322695VDV
CSB-EP322695VDV
CSB-BP322695VDV
CSB-MP322695VDV
CSB-EP322695VDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dunaliella salina Chlorophyll a-b binding protein of LHCII type I, chloroplastic, partial CSB-YP322696DMW
CSB-EP322696DMW
CSB-BP322696DMW
CSB-MP322696DMW
CSB-EP322696DMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Nef (nef) CSB-YP322697HKS
CSB-EP322697HKS
CSB-BP322697HKS
CSB-MP322697HKS
CSB-EP322697HKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Envelope glycoprotein gp160 (env), partial CSB-YP322698HKS
CSB-EP322698HKS
CSB-BP322698HKS
CSB-MP322698HKS
CSB-EP322698HKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Gag polyprotein (gag), partial CSB-YP322699HMB
CSB-EP322699HMB
CSB-BP322699HMB
CSB-MP322699HMB
CSB-EP322699HMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Protein Tat CSB-YP322700HKS
CSB-EP322700HKS
CSB-BP322700HKS
CSB-MP322700HKS
CSB-EP322700HKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 1 group M subtype B Virion infectivity factor (vif) CSB-YP322701HKZ
CSB-EP322701HKZ
CSB-BP322701HKZ
CSB-MP322701HKZ
CSB-EP322701HKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human respiratory syncytial virus A Major surface glycoprotein G (G), partial CSB-YP322702HPP1
CSB-EP322702HPP1
CSB-BP322702HPP1
CSB-MP322702HPP1
CSB-EP322702HPP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II D2 protein (psbD1), partial CSB-YP322703FQA1
CSB-EP322703FQA1
CSB-BP322703FQA1
CSB-MP322703FQA1
CSB-EP322703FQA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Benzoylformate decarboxylase (mdlC), partial CSB-YP322704FFZ
CSB-EP322704FFZ
CSB-BP322704FFZ
CSB-MP322704FFZ
CSB-EP322704FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Homeotic protein NRL-19 CSB-YP322705XBE
CSB-EP322705XBE
CSB-BP322705XBE
CSB-MP322705XBE
CSB-EP322705XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine infectious anemia virus Protein Rev (rev) CSB-YP322706EFZ
CSB-EP322706EFZ
CSB-BP322706EFZ
CSB-MP322706EFZ
CSB-EP322706EFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Frd operon uncharacterized protein C CSB-YP322707EZA
CSB-EP322707EZA
CSB-BP322707EZA
CSB-MP322707EZA
CSB-EP322707EZA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variant surface glycoprotein WRATAT A CSB-YP322708TQX
CSB-EP322708TQX
CSB-BP322708TQX
CSB-MP322708TQX
CSB-EP322708TQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variant surface glycoprotein WRATAT B CSB-YP322709TQX
CSB-EP322709TQX
CSB-BP322709TQX
CSB-MP322709TQX
CSB-EP322709TQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papaya mosaic potexvirus Movement and silencing protein TGBp1 (ORF2) CSB-YP322710PBN
CSB-EP322710PBN
CSB-BP322710PBN
CSB-MP322710PBN
CSB-EP322710PBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papaya mosaic potexvirus Uncharacterized 14.1 kDa protein in ORF1 coding strand CSB-YP322711PBN
CSB-EP322711PBN
CSB-BP322711PBN
CSB-MP322711PBN
CSB-EP322711PBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli PTS system fructose-specific EIIBC component (fruA), partial CSB-YP322712ENV
CSB-EP322712ENV
CSB-BP322712ENV
CSB-MP322712ENV
CSB-EP322712ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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