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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Vaccinia virus Uncharacterized 7.9 kDa protein (A ORF N) CSB-YP323243VAA
CSB-EP323243VAA
CSB-BP323243VAA
CSB-MP323243VAA
CSB-EP323243VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Serine proteinase inhibitor 1 (SPI-1) CSB-YP323244VAA
CSB-EP323244VAA
CSB-BP323244VAA
CSB-MP323244VAA
CSB-EP323244VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Transcript termination protein A18 (A18R) CSB-YP323245VAA
CSB-EP323245VAA
CSB-BP323245VAA
CSB-MP323245VAA
CSB-EP323245VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Late protein H7 (H7R), partial CSB-YP323246VAA1
CSB-EP323246VAA1
CSB-BP323246VAA1
CSB-MP323246VAA1
CSB-EP323246VAA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 11.2 kDa protein (B ORF G) CSB-YP323247VAA
CSB-EP323247VAA
CSB-BP323247VAA
CSB-MP323247VAA
CSB-EP323247VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 7.3 kDa protein (D ORF C) CSB-YP323248VAA
CSB-EP323248VAA
CSB-BP323248VAA
CSB-MP323248VAA
CSB-EP323248VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 11.6 kDa protein (F ORF C) CSB-YP323249VAA
CSB-EP323249VAA
CSB-BP323249VAA
CSB-MP323249VAA
CSB-EP323249VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized 14.3 kDa protein (G ORF A) CSB-YP323250VAA
CSB-EP323250VAA
CSB-BP323250VAA
CSB-MP323250VAA
CSB-EP323250VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui Uncharacterized protein rrnAC1612 (rrnAC1612) CSB-YP323251HTJ
CSB-EP323251HTJ
CSB-BP323251HTJ
CSB-MP323251HTJ
CSB-EP323251HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Anthranilate synthase component 1 (trpE) CSB-YP323252FFZ
CSB-EP323252FFZ
CSB-BP323252FFZ
CSB-MP323252FFZ
CSB-EP323252FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa PQB biosynthetic 3-oxoacyl-[acyl-carrier-protein] synthase III (pqsD) CSB-YP323253EZX
CSB-EP323253EZX
CSB-BP323253EZX
CSB-MP323253EZX
CSB-EP323253EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type-2 restriction enzyme HinfI (hinfIR) CSB-YP323254HSZ
CSB-EP323254HSZ
CSB-BP323254HSZ
CSB-MP323254HSZ
CSB-EP323254HSZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bluetongue virus 1 Core protein VP3 (S3), partial CSB-YP323255BHN
CSB-EP323255BHN
CSB-BP323255BHN
CSB-MP323255BHN
CSB-EP323255BHN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Sex-specific storage-protein 2 (SP2), partial CSB-YP323256BTT
CSB-EP323256BTT
CSB-BP323256BTT
CSB-MP323256BTT
CSB-EP323256BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase molybdenum-iron protein alpha chain (nifD) CSB-YP323257MSC
CSB-EP323257MSC
CSB-BP323257MSC
CSB-MP323257MSC
CSB-EP323257MSC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. Nitrogen fixation protein nifU (nifU) CSB-YP323258NHR
CSB-EP323258NHR
CSB-BP323258NHR
CSB-MP323258NHR
CSB-EP323258NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Major envelope protein (F13L) CSB-YP323260VAA
CSB-BP323260VAA
CSB-MP323260VAA
CSB-EP323260VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein N2 (N2L) CSB-YP323261VAA
CSB-EP323261VAA
CSB-BP323261VAA
CSB-MP323261VAA
CSB-EP323261VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Protein 10 CSB-YP323262GU
CSB-EP323262GU
CSB-BP323262GU
CSB-MP323262GU
CSB-EP323262GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pilin gene-inverting protein (piv) CSB-YP323263MHZ
CSB-EP323263MHZ
CSB-BP323263MHZ
CSB-MP323263MHZ
CSB-EP323263MHZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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