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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mouse Mast cell protease 3 (Mcpt3) CSB-YP324271MO
CSB-EP324271MO
CSB-BP324271MO
CSB-MP324271MO
CSB-EP324271MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hypocrea jecorina Orotate phosphoribosyltransferase (ura5) CSB-YP324272HYE
CSB-EP324272HYE
CSB-BP324272HYE
CSB-MP324272HYE
CSB-EP324272HYE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio vulgaris Periplasmic [NiFe] hydrogenase large subunit (hydB), partial CSB-YP324273DJR
CSB-EP324273DJR
CSB-BP324273DJR
CSB-MP324273DJR
CSB-EP324273DJR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops flavoviridis Disintegrin triflavin CSB-YP324274EZB
CSB-EP324274EZB
CSB-BP324274EZB
CSB-MP324274EZB
CSB-EP324274EZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP324275FFT
CSB-EP324275FFT
CSB-BP324275FFT
CSB-MP324275FFT
CSB-EP324275FFT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional regulator rafR (rafR) CSB-YP324276ENL
CSB-EP324276ENL
CSB-BP324276ENL
CSB-MP324276ENL
CSB-EP324276ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Columba livia Hemoglobin subunit alpha-A (HBAA) CSB-YP324277DVK
CSB-EP324277DVK
CSB-BP324277DVK
CSB-MP324277DVK
CSB-EP324277DVK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Variable large protein 21 (vlp21) CSB-YP324278BIP
CSB-EP324278BIP
CSB-BP324278BIP
CSB-MP324278BIP
CSB-EP324278BIP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Small, acid-soluble spore protein C2 (sspC2) CSB-YP324280CMB
CSB-EP324280CMB
CSB-BP324280CMB
CSB-MP324280CMB
CSB-EP324280CMB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anabaena sp. Ferredoxin--NADP reductase (petH) CSB-YP324281BYM
CSB-EP324281BYM
CSB-BP324281BYM
CSB-MP324281BYM
CSB-EP324281BYM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chironomus thummi thummi Histone H1 CSB-YP324282CIR
CSB-EP324282CIR
CSB-BP324282CIR
CSB-MP324282CIR
CSB-EP324282CIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tachypleus tridentatus Proclotting enzyme, partial CSB-YP324283TAH
CSB-EP324283TAH
CSB-BP324283TAH
CSB-MP324283TAH
CSB-EP324283TAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c, sodium ion specific (atpE), partial CSB-YP324284PSI1
CSB-EP324284PSI1
CSB-BP324284PSI1
CSB-MP324284PSI1
CSB-EP324284PSI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (sdh2) CSB-YP324285SXV
CSB-EP324285SXV
CSB-BP324285SXV
CSB-MP324285SXV
CSB-EP324285SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Precorrin-6Y C (5,15)-methyltransferase [decarboxylating] CSB-YP324286PUH
CSB-EP324286PUH
CSB-BP324286PUH
CSB-MP324286PUH
CSB-EP324286PUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sorghum bicolor Defensin-like protein 1 CSB-YP324287FJS
CSB-EP324287FJS
CSB-BP324287FJS
CSB-MP324287FJS
CSB-EP324287FJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Squash leaf curl virus Movement protein BC1 (BC1, BL1) CSB-YP324288SKQ
CSB-EP324288SKQ
CSB-BP324288SKQ
CSB-MP324288SKQ
CSB-EP324288SKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Abutilon mosaic virus Capsid protein (AR1, AV1) CSB-YP324289AAE
CSB-EP324289AAE
CSB-BP324289AAE
CSB-MP324289AAE
CSB-EP324289AAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Abutilon mosaic virus Nuclear shuttle protein (BR1, BV1) CSB-YP324290AAE
CSB-EP324290AAE
CSB-BP324290AAE
CSB-MP324290AAE
CSB-EP324290AAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Isocitrate dehydrogenase [NADP], mitochondrial (IDP1) CSB-YP324291SVG
CSB-EP324291SVG
CSB-BP324291SVG
CSB-MP324291SVG
CSB-EP324291SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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