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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Uncharacterized protein 1b (ORF Ib) CSB-YP324479SIZ
CSB-EP324479SIZ
CSB-BP324479SIZ
CSB-MP324479SIZ
CSB-EP324479SIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Repetitive proline-rich cell wall protein 3 (PRP3) CSB-YP324480GGV
CSB-EP324480GGV
CSB-BP324480GGV
CSB-MP324480GGV
CSB-EP324480GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP324481IBT
CSB-EP324481IBT
CSB-BP324481IBT
CSB-MP324481IBT
CSB-EP324481IBT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP324482IMM
CSB-EP324482IMM
CSB-BP324482IMM
CSB-MP324482IMM
CSB-EP324482IMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parachlorella kessleri H (+)/hexose cotransporter 1, partial CSB-YP324483PBY
CSB-EP324483PBY
CSB-BP324483PBY
CSB-MP324483PBY
CSB-EP324483PBY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paeniclostridium sordellii Sialidase CSB-EP324484CMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 3-isopropylmalate dehydratase large subunit 1 (leuC1) CSB-YP324485SXB
CSB-EP324485SXB
CSB-BP324485SXB
CSB-MP324485SXB
CSB-EP324485SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Deoxyguanosinetriphosphate triphosphohydrolase (dgt) CSB-YP324486ENV
CSB-EP324486ENV
CSB-BP324486ENV
CSB-MP324486ENV
CSB-EP324486ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type II secretion system protein I (pulI) CSB-YP324487KBG
CSB-EP324487KBG
CSB-BP324487KBG
CSB-MP324487KBG
CSB-EP324487KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus 30S ribosomal protein S13 (rpsM) CSB-YP324488GFM
CSB-EP324488GFM
CSB-BP324488GFM
CSB-MP324488GFM
CSB-EP324488GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L2, cyanelle (rpl2) CSB-YP324489DZX
CSB-EP324489DZX
CSB-BP324489DZX
CSB-MP324489DZX
CSB-EP324489DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Surface antigen CRP170 CSB-EP324490GFW
CSB-BP324490GFW
CSB-MP324490GFW
CSB-EP324490GFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Siroheme biosynthesis protein MET8 (MET8) CSB-YP324491SVG
CSB-EP324491SVG
CSB-BP324491SVG
CSB-MP324491SVG
CSB-EP324491SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bungarus multicinctus Long neurotoxin homolog CSB-YP324492BXN
CSB-EP324492BXN
CSB-BP324492BXN
CSB-MP324492BXN
CSB-EP324492BXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exoglucanase 1 (CBH-1), partial CSB-YP324493HQM
CSB-EP324493HQM
CSB-BP324493HQM
CSB-MP324493HQM
CSB-EP324493HQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype B Protein Vpr (vpr) CSB-YP324494HMD
CSB-EP324494HMD
CSB-BP324494HMD
CSB-MP324494HMD
CSB-EP324494HMD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi15 Early protein GP17 (17) CSB-YP324495BBB
CSB-EP324495BBB
CSB-BP324495BBB
CSB-MP324495BBB
CSB-EP324495BBB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apis mellifera Homeobox protein H90 CSB-YP324496DNK
CSB-EP324496DNK
CSB-BP324496DNK
CSB-MP324496DNK
CSB-EP324496DNK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Psammechinus miliaris Histone H1 CSB-YP324497PTB
CSB-EP324497PTB
CSB-BP324497PTB
CSB-MP324497PTB
CSB-EP324497PTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus vannielii Uncharacterized protein Mevan_1275 (Mevan_1275) CSB-YP324498MNS
CSB-EP324498MNS
CSB-BP324498MNS
CSB-MP324498MNS
CSB-EP324498MNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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