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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Proteus mirabilis Urease accessory protein UreF (ureF) CSB-YP323488EYZ
CSB-EP323488EYZ
CSB-BP323488EYZ
CSB-MP323488EYZ
CSB-EP323488EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype A2 subtype adw2 Large envelope protein (S), partial CSB-YP323489HDO1
CSB-EP323489HDO1
CSB-BP323489HDO1
CSB-MP323489HDO1
CSB-EP323489HDO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratitis capitata Chorion protein S36 (Cp36) CSB-YP323490DRF
CSB-EP323490DRF
CSB-BP323490DRF
CSB-MP323490DRF
CSB-EP323490DRF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GTPase-activating protein SAC7 (SAC7), partial CSB-YP323491SVG
CSB-EP323491SVG
CSB-BP323491SVG
CSB-MP323491SVG
CSB-EP323491SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Heterogeneous nuclear ribonucleoprotein C (Hnrnpc) CSB-YP323492RA
CSB-EP323492RA
CSB-BP323492RA
CSB-MP323492RA
CSB-EP323492RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein IRL3 CSB-YP323493HWV
CSB-EP323493HWV
CSB-BP323493HWV
CSB-MP323493HWV
CSB-EP323493HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Viral interleukin-10 homolog (UL111A) CSB-YP323494HWV
CSB-BP323494HWV
CSB-MP323494HWV
CSB-EP323494HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cyclin-dependent protein kinase PHO85 (PHO85) CSB-YP323495SVG
CSB-EP323495SVG
CSB-BP323495SVG
CSB-MP323495SVG
CSB-EP323495SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate phosphoribosyltransferase (trpD) CSB-YP323496LMH
CSB-EP323496LMH
CSB-BP323496LMH
CSB-MP323496LMH
CSB-EP323496LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Duck hepatitis B virus Large envelope protein (S), partial CSB-YP323497DHG1
CSB-EP323497DHG1
CSB-BP323497DHG1
CSB-MP323497DHG1
CSB-EP323497DHG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia muridarum 10 kDa chaperonin (groS) CSB-YP323498CIU
CSB-EP323498CIU
CSB-BP323498CIU
CSB-MP323498CIU
CSB-EP323498CIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type I restriction enzyme EcoprrI (prrD) CSB-YP323499ENL
CSB-EP323499ENL
CSB-BP323499ENL
CSB-MP323499ENL
CSB-EP323499ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Photosystem I reaction center subunit IX (psaJ), partial CSB-YP323500FKI
CSB-EP323500FKI
CSB-BP323500FKI
CSB-MP323500FKI
CSB-EP323500FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Vpr (vpr) CSB-YP323501SGW
CSB-EP323501SGW
CSB-BP323501SGW
CSB-MP323501SGW
CSB-EP323501SGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S7, cyanelle (rps7) CSB-YP323502DZX
CSB-EP323502DZX
CSB-BP323502DZX
CSB-MP323502DZX
CSB-EP323502DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 DNA-packaging protein Gp16 (16) CSB-YP323503EDZ
CSB-EP323503EDZ
CSB-BP323503EDZ
CSB-MP323503EDZ
CSB-EP323503EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP323504ZAX1
CSB-EP323504ZAX1
CSB-BP323504ZAX1
CSB-MP323504ZAX1
CSB-EP323504ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized protein 16 (VACWR016) CSB-YP323505VAI
CSB-EP323505VAI
CSB-BP323505VAI
CSB-MP323505VAI
CSB-EP323505VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C8L (VACWR020) CSB-YP323506VAI
CSB-EP323506VAI
CSB-BP323506VAI
CSB-MP323506VAI
CSB-EP323506VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Kelch repeat protein C2 (VACWR026), partial CSB-YP323507VAI
CSB-EP323507VAI
CSB-BP323507VAI
CSB-MP323507VAI
CSB-EP323507VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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