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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. Uncharacterized PKHD-type hydroxylase sll0191 (sll0191) CSB-YP324312SSQ
CSB-EP324312SSQ
CSB-BP324312SSQ
CSB-MP324312SSQ
CSB-EP324312SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable reductase (P100/11E) CSB-YP324314LOG
CSB-EP324314LOG
CSB-BP324314LOG
CSB-MP324314LOG
CSB-EP324314LOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato black ring virus Satellite RNA 48 kDa protein CSB-YP324315TFU
CSB-EP324315TFU
CSB-BP324315TFU
CSB-MP324315TFU
CSB-EP324315TFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato black ring virus Satellite RNA 48 kDa protein CSB-YP324316TFW
CSB-EP324316TFW
CSB-BP324316TFW
CSB-MP324316TFW
CSB-EP324316TFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guinea pig Caltrin-like protein 2 CSB-YP324318GU
CSB-EP324318GU
CSB-BP324318GU
CSB-MP324318GU
CSB-EP324318GU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taenia taeniaeformis Oncosphere antigen A (ONCA) CSB-YP324319TAL
CSB-EP324319TAL
CSB-BP324319TAL
CSB-MP324319TAL
CSB-EP324319TAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcription regulatory protein SNF2 (SNF2), partial CSB-YP324320SVG
CSB-EP324320SVG
CSB-BP324320SVG
CSB-MP324320SVG
CSB-EP324320SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cepacia Lipase chaperone (lifO), partial CSB-YP324321BXR1
CSB-EP324321BXR1
CSB-BP324321BXR1
CSB-MP324321BXR1
CSB-EP324321BXR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Hemagglutinin (HA), partial CSB-YP324322IJX
CSB-EP324322IJX
CSB-BP324322IJX
CSB-MP324322IJX
CSB-EP324322IJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate phosphoribosyltransferase (trpD) CSB-YP324323VFE
CSB-EP324323VFE
CSB-BP324323VFE
CSB-MP324323VFE
CSB-EP324323VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthranilate synthase component 1 (trpE), partial CSB-YP324324VFE
CSB-EP324324VFE
CSB-BP324324VFE
CSB-MP324324VFE
CSB-EP324324VFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Trifunctional purine biosynthetic protein adenosine-3 (GART), partial CSB-YP324325HU
CSB-BP324325HU
CSB-MP324325HU
CSB-EP324325HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant TraT complement resistance protein (traT) CSB-YP324326SWX
CSB-EP324326SWX
CSB-BP324326SWX
CSB-MP324326SWX
CSB-EP324326SWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Small hydrophobic protein (SH), partial CSB-YP324328MJI
CSB-EP324328MJI
CSB-BP324328MJI
CSB-MP324328MJI
CSB-EP324328MJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Small hydrophobic protein (SH), partial CSB-YP324329MJN
CSB-EP324329MJN
CSB-BP324329MJN
CSB-MP324329MJN
CSB-EP324329MJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peanut stunt virus Capsid protein (ORF3b) CSB-YP324330PDZ
CSB-EP324330PDZ
CSB-BP324330PDZ
CSB-MP324330PDZ
CSB-EP324330PDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maize rough dwarf virus Probable non-structural 36.3 kDa protein (S6) CSB-YP324331MBI
CSB-EP324331MBI
CSB-BP324331MBI
CSB-MP324331MBI
CSB-EP324331MBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Discopyge ommata Ras-related protein O-Krev CSB-YP324332DES
CSB-EP324332DES
CSB-BP324332DES
CSB-MP324332DES
CSB-EP324332DES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Diplobatis ommata Ras-related protein ORAB-1 CSB-YP324333DES
CSB-EP324333DES
CSB-BP324333DES
CSB-MP324333DES
CSB-EP324333DES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Discopyge ommata Ras-related protein Rab-8 CSB-YP324334DES
CSB-EP324334DES
CSB-BP324334DES
CSB-MP324334DES
CSB-EP324334DES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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