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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Methanothermus fervidus Malate/L-sulfolactate dehydrogenase (mdh) CSB-YP324522MST
CSB-EP324522MST
CSB-BP324522MST
CSB-MP324522MST
CSB-EP324522MST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus polyphyllus Protein PPLZ12 (PPLZ12) CSB-YP324523LKJ
CSB-EP324523LKJ
CSB-BP324523LKJ
CSB-MP324523LKJ
CSB-EP324523LKJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridioides difficile Toxin A (toxA), partial CSB-YP324524DUN
CSB-BP324524DUN
CSB-MP324524DUN
CSB-EP324524DUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 40S ribosomal protein S11-1 (RPS11A) CSB-YP324525DOA
CSB-EP324525DOA
CSB-BP324525DOA
CSB-MP324525DOA
CSB-EP324525DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Neuraminidase (NA), partial CSB-YP324526IJN
CSB-EP324526IJN
CSB-BP324526IJN
CSB-MP324526IJN
CSB-EP324526IJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Glycoprotein NB (NB), partial CSB-YP324527IJY1
CSB-EP324527IJY1
CSB-BP324527IJY1
CSB-MP324527IJY1
CSB-EP324527IJY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Endoglucanase H (celH), partial CSB-YP324528DUT
CSB-EP324528DUT
CSB-BP324528DUT
CSB-MP324528DUT
CSB-EP324528DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meles meles Double-headed protease inhibitor, submandibular gland CSB-YP324529MDP
CSB-EP324529MDP
CSB-BP324529MDP
CSB-MP324529MDP
CSB-EP324529MDP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Histidinol dehydrogenase (hisD) CSB-YP324530FOB
CSB-EP324530FOB
CSB-BP324530FOB
CSB-MP324530FOB
CSB-EP324530FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Phosphoribosyl isomerase A (priA) CSB-YP324531FOB
CSB-EP324531FOB
CSB-BP324531FOB
CSB-MP324531FOB
CSB-EP324531FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein 3 (anfH) CSB-YP324532DPX
CSB-EP324532DPX
CSB-BP324532DPX
CSB-MP324532DPX
CSB-EP324532DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 23 kDa protein CSB-YP324533PDW
CSB-EP324533PDW
CSB-BP324533PDW
CSB-MP324533PDW
CSB-EP324533PDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabies virus Large structural protein (L), partial CSB-YP324534RAJ
CSB-EP324534RAJ
CSB-BP324534RAJ
CSB-MP324534RAJ
CSB-EP324534RAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 120.7 kDa protein in NOF-FB transposable element (NOF), partial CSB-YP324535DLU
CSB-EP324535DLU
CSB-BP324535DLU
CSB-MP324535DLU
CSB-EP324535DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heteractis crispa Kunitz-type trypsin inhibitor IV CSB-YP324536HWE
CSB-EP324536HWE
CSB-BP324536HWE
CSB-MP324536HWE
CSB-EP324536HWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lathyrus sphaericus Lectin CSB-YP324537LCX
CSB-EP324537LCX
CSB-BP324537LCX
CSB-MP324537LCX
CSB-EP324537LCX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Formate hydrogenlyase subunit 4 (hycD), partial CSB-YP324539ENV
CSB-EP324539ENV
CSB-BP324539ENV
CSB-MP324539ENV
CSB-EP324539ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore germination protein gerD (gerD) CSB-YP324540BRJ
CSB-EP324540BRJ
CSB-BP324540BRJ
CSB-MP324540BRJ
CSB-EP324540BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemolysin transporter protein hpmB (hpmB), partial CSB-YP324541EYY
CSB-EP324541EYY
CSB-BP324541EYY
CSB-MP324541EYY
CSB-EP324541EYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphocarrier protein HPr (ptsH) CSB-YP324542KBG
CSB-EP324542KBG
CSB-BP324542KBG
CSB-MP324542KBG
CSB-EP324542KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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