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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Trichoderma koningii Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd2) CSB-YP323798THY
CSB-EP323798THY
CSB-BP323798THY
CSB-MP323798THY
CSB-EP323798THY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Outer surface protein B (ospB) CSB-YP323799BUD
CSB-EP323799BUD
CSB-BP323799BUD
CSB-MP323799BUD
CSB-EP323799BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sendai virus Matrix protein (M) CSB-YP323800SEX
CSB-EP323800SEX
CSB-BP323800SEX
CSB-MP323800SEX
CSB-EP323800SEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human immunodeficiency virus type 2 subtype A Gag polyprotein (gag), partial CSB-YP323801HLW
CSB-EP323801HLW
CSB-BP323801HLW
CSB-MP323801HLW
CSB-EP323801HLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dengue virus type 1 Genome polyprotein, partial CSB-YP323802DBV
CSB-EP323802DBV
CSB-BP323802DBV
CSB-MP323802DBV
CSB-EP323802DBV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Organ-specific protein S2 CSB-YP323803EWE
CSB-EP323803EWE
CSB-BP323803EWE
CSB-MP323803EWE
CSB-EP323803EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrullus lanatus Malate dehydrogenase, mitochondrial (MMDH) CSB-YP323804CKU
CSB-EP323804CKU
CSB-BP323804CKU
CSB-MP323804CKU
CSB-EP323804CKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 50S ribosomal protein L2, chloroplastic (rpl2-A) CSB-YP323805ZAX
CSB-EP323805ZAX
CSB-BP323805ZAX
CSB-MP323805ZAX
CSB-EP323805ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Protein virB6 (virB6), partial CSB-YP323806AYS1
CSB-EP323806AYS1
CSB-BP323806AYS1
CSB-MP323806AYS1
CSB-EP323806AYS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli A/G-specific adenine glycosylase (mutY) CSB-YP323807ENV
CSB-EP323807ENV
CSB-BP323807ENV
CSB-MP323807ENV
CSB-EP323807ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Fimbrial protein (fimA) CSB-YP323808DDR
CSB-EP323808DDR
CSB-BP323808DDR
CSB-MP323808DDR
CSB-EP323808DDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Fimbrial assembly protein, serogroup E2 (fimB) CSB-YP323809DDR
CSB-EP323809DDR
CSB-BP323809DDR
CSB-MP323809DDR
CSB-EP323809DDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica oleracea S-locus-specific glycoprotein S13 (SLSG) CSB-YP323810BWE
CSB-EP323810BWE
CSB-BP323810BWE
CSB-MP323810BWE
CSB-EP323810BWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gamma-glutamyl phosphate reductase (proA) CSB-YP323811SYN
CSB-EP323811SYN
CSB-BP323811SYN
CSB-MP323811SYN
CSB-EP323811SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cell division protein FtsZ (ftsZ) CSB-YP323812BRJ
CSB-EP323812BRJ
CSB-BP323812BRJ
CSB-MP323812BRJ
CSB-EP323812BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum Glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC) CSB-YP323813MRC
CSB-EP323813MRC
CSB-BP323813MRC
CSB-MP323813MRC
CSB-EP323813MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Arginine repressor (argR) CSB-YP323814BRJ
CSB-EP323814BRJ
CSB-BP323814BRJ
CSB-MP323814BRJ
CSB-EP323814BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Serine-protein kinase rsbW (rsbW) CSB-YP323815BRJ
CSB-EP323815BRJ
CSB-BP323815BRJ
CSB-MP323815BRJ
CSB-EP323815BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus awamori Aspartic protease pep1 (pep1) CSB-YP323817APL
CSB-EP323817APL
CSB-BP323817APL
CSB-MP323817APL
CSB-EP323817APL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse T-cell ecto-ADP-ribosyltransferase 1 (Art2a) CSB-YP323818MO
CSB-EP323818MO
CSB-BP323818MO
CSB-MP323818MO
CSB-EP323818MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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