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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rotavirus A Inner capsid protein VP2, partial CSB-YP323778RGK
CSB-EP323778RGK
CSB-BP323778RGK
CSB-MP323778RGK
CSB-EP323778RGK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A RNA-directed RNA polymerase, partial CSB-YP323779RGJ
CSB-EP323779RGJ
CSB-BP323779RGJ
CSB-MP323779RGJ
CSB-EP323779RGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bleomycin resistance protein (ble) CSB-YP323780SMD
CSB-EP323780SMD
CSB-BP323780SMD
CSB-MP323780SMD
CSB-EP323780SMD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthonomus grandis Protamine CSB-YP323781ALK
CSB-EP323781ALK
CSB-BP323781ALK
CSB-MP323781ALK
CSB-EP323781ALK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Elongation factor 1-alpha, oocyte form CSB-YP323782XBE
CSB-EP323782XBE
CSB-BP323782XBE
CSB-MP323782XBE
CSB-EP323782XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Acidic endochitinase Q CSB-YP323783NHE
CSB-EP323783NHE
CSB-BP323783NHE
CSB-MP323783NHE
CSB-EP323783NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Movement protein (ORF4) CSB-YP323784PQU
CSB-EP323784PQU
CSB-BP323784PQU
CSB-MP323784PQU
CSB-EP323784PQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Superoxide dismutase [Fe] (chrC) CSB-YP323785RJG
CSB-EP323785RJG
CSB-BP323785RJG
CSB-MP323785RJG
CSB-EP323785RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 37S ribosomal protein PET123, mitochondrial (PET123) CSB-YP323786SVG
CSB-EP323786SVG
CSB-BP323786SVG
CSB-MP323786SVG
CSB-EP323786SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucurbita maxima Nitrate reductase [NADH], partial CSB-YP323787DZK
CSB-EP323787DZK
CSB-BP323787DZK
CSB-MP323787DZK
CSB-EP323787DZK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Satellite tobacco mosaic virus 6.8 kDa protein CSB-YP323788SCW
CSB-EP323788SCW
CSB-BP323788SCW
CSB-MP323788SCW
CSB-EP323788SCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Triplex capsid protein VP19C (UL38) CSB-YP323789HJI
CSB-EP323789HJI
CSB-BP323789HJI
CSB-MP323789HJI
CSB-EP323789HJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human adenovirus B serotype 7 Early E3 7.7 kDa protein, partial CSB-YP323790HII1
CSB-EP323790HII1
CSB-BP323790HII1
CSB-MP323790HII1
CSB-EP323790HII1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein RibT (ribT) CSB-YP323791BRJ
CSB-EP323791BRJ
CSB-BP323791BRJ
CSB-MP323791BRJ
CSB-EP323791BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rubrivivax gelatinosus Uptake hydrogenase large subunit (hupB), partial CSB-YP323792RHU
CSB-EP323792RHU
CSB-BP323792RHU
CSB-MP323792RHU
CSB-EP323792RHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus tschawytscha Pro-MCH 1 (mch1) CSB-YP323793OBK
CSB-EP323793OBK
CSB-BP323793OBK
CSB-MP323793OBK
CSB-EP323793OBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Opsin Rh4 (Rh4), partial CSB-YP323794DMP1
CSB-EP323794DMP1
CSB-BP323794DMP1
CSB-MP323794DMP1
CSB-EP323794DMP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas moewusii Light-independent protochlorophyllide reductase subunit B (chlB), partial CSB-YP323795CIX
CSB-EP323795CIX
CSB-BP323795CIX
CSB-MP323795CIX
CSB-EP323795CIX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Ecdysone-induced protein 75B, isoforms C/D (Eip75B), partial CSB-YP323796DLU
CSB-EP323796DLU
CSB-BP323796DLU
CSB-MP323796DLU
CSB-EP323796DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dendroaspis angusticeps Synergistic-like venom protein CSB-YP323797DBG
CSB-EP323797DBG
CSB-BP323797DBG
CSB-MP323797DBG
CSB-EP323797DBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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