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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dictyostelium discoideum Myosin IA heavy chain (myoA), partial CSB-YP324417DKK
CSB-EP324417DKK
CSB-BP324417DKK
CSB-MP324417DKK
CSB-EP324417DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice dwarf virus Outer capsid protein P3, partial CSB-YP324418REN
CSB-EP324418REN
CSB-BP324418REN
CSB-MP324418REN
CSB-EP324418REN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pseudofirmus ATP synthase protein I (atpI), partial CSB-YP324419BRB1
CSB-EP324419BRB1
CSB-BP324419BRB1
CSB-MP324419BRB1
CSB-EP324419BRB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pseudofirmus ATP synthase epsilon chain (atpC) CSB-YP324420BRB
CSB-EP324420BRB
CSB-BP324420BRB
CSB-MP324420BRB
CSB-EP324420BRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pseudofirmus ATP synthase subunit c (atpE), partial CSB-YP324421BRB1
CSB-EP324421BRB1
CSB-BP324421BRB1
CSB-MP324421BRB1
CSB-EP324421BRB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Glycoprotein K (gK), partial CSB-YP324422HJX1
CSB-EP324422HJX1
CSB-BP324422HJX1
CSB-MP324422HJX1
CSB-EP324422HJX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helix aspersa Non-neuronal cytoplasmic intermediate filament protein, partial CSB-YP324423HVH
CSB-EP324423HVH
CSB-BP324423HVH
CSB-MP324423HVH
CSB-EP324423HVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Protein umuC (umuC) CSB-YP324424SXB
CSB-EP324424SXB
CSB-BP324424SXB
CSB-MP324424SXB
CSB-EP324424SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cysteine proteinase (TP03_0285) CSB-YP324425TCK
CSB-EP324425TCK
CSB-BP324425TCK
CSB-MP324425TCK
CSB-EP324425TCK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage P2 Major tail sheath protein (FI) CSB-YP324426EDE
CSB-EP324426EDE
CSB-BP324426EDE
CSB-MP324426EDE
CSB-EP324426EDE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paenibacillus polymyxa Beta-glucosidase B (bglB) CSB-YP324427EQK
CSB-EP324427EQK
CSB-BP324427EQK
CSB-MP324427EQK
CSB-EP324427EQK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glyceraldehyde-3-phosphate dehydrogenase, glycosomal CSB-YP324429TQV
CSB-EP324429TQV
CSB-BP324429TQV
CSB-MP324429TQV
CSB-EP324429TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase CHL1 (CHL1), partial CSB-YP324430SVG
CSB-EP324430SVG
CSB-BP324430SVG
CSB-MP324430SVG
CSB-EP324430SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colicin V secretion protein CvaA (cvaA), partial CSB-YP324431ENL
CSB-EP324431ENL
CSB-BP324431ENL
CSB-MP324431ENL
CSB-EP324431ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Colicin-V (cvaC) CSB-YP324432ENL
CSB-EP324432ENL
CSB-BP324432ENL
CSB-MP324432ENL
CSB-EP324432ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African cassava mosaic virus Movement protein BC1 (BC1, BL1) CSB-YP324433ADW
CSB-EP324433ADW
CSB-BP324433ADW
CSB-MP324433ADW
CSB-EP324433ADW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beet curly top virus Replication enhancer (C3, L3) CSB-YP324435BFX
CSB-EP324435BFX
CSB-BP324435BFX
CSB-MP324435BFX
CSB-EP324435BFX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant BK polyomavirus Large T antigen, partial CSB-YP324436BGZ
CSB-EP324436BGZ
CSB-BP324436BGZ
CSB-MP324436BGZ
CSB-EP324436BGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein mcrC (mcrC) CSB-YP324437ENV
CSB-EP324437ENV
CSB-BP324437ENV
CSB-MP324437ENV
CSB-EP324437ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c (atpE), partial CSB-YP324438RMA1
CSB-EP324438RMA1
CSB-BP324438RMA1
CSB-MP324438RMA1
CSB-EP324438RMA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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