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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nitrogenase vanadium-iron protein beta chain (vnfK) CSB-YP323733DPX
CSB-EP323733DPX
CSB-BP323733DPX
CSB-MP323733DPX
CSB-EP323733DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Volvox carteri Histone H2A-IV CSB-YP323734VDT
CSB-EP323734VDT
CSB-BP323734VDT
CSB-MP323734VDT
CSB-EP323734VDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thrombospondin-related anonymous protein (TRAP), partial CSB-YP323735PLO
CSB-EP323735PLO
CSB-BP323735PLO
CSB-MP323735PLO
CSB-EP323735PLO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ovine maedi visna related virus Virion infectivity factor (vif) CSB-YP323736OCW
CSB-EP323736OCW
CSB-BP323736OCW
CSB-MP323736OCW
CSB-EP323736OCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein rhsA (rhsA), partial CSB-YP323737ENV
CSB-EP323737ENV
CSB-BP323737ENV
CSB-MP323737ENV
CSB-EP323737ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequence element IS640 (istA) CSB-YP323739SGH
CSB-EP323739SGH
CSB-BP323739SGH
CSB-MP323739SGH
CSB-EP323739SGH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agglutinin receptor (ssp5), partial CSB-YP323740SML
CSB-EP323740SML
CSB-BP323740SML
CSB-MP323740SML
CSB-EP323740SML-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare Alpha-amylase/trypsin inhibitor CSB-YP323741HWQ
CSB-EP323741HWQ
CSB-BP323741HWQ
CSB-MP323741HWQ
CSB-EP323741HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP323742ICS
CSB-EP323742ICS
CSB-BP323742ICS
CSB-MP323742ICS
CSB-EP323742ICS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nucleoprotein (NP) CSB-YP323743IGT
CSB-EP323743IGT
CSB-BP323743IGT
CSB-MP323743IGT
CSB-EP323743IGT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Hemagglutinin (HA), partial CSB-YP323744IDV
CSB-EP323744IDV
CSB-BP323744IDV
CSB-MP323744IDV
CSB-EP323744IDV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP323745IEY
CSB-EP323745IEY
CSB-BP323745IEY
CSB-MP323745IEY
CSB-EP323745IEY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 13 kDa prolamin C (PROLM25) CSB-YP323746OFG
CSB-BP323746OFG
CSB-MP323746OFG
CSB-EP323746OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Spheroidene monooxygenase (crtA) CSB-YP323747RLD
CSB-EP323747RLD
CSB-BP323747RLD
CSB-MP323747RLD
CSB-EP323747RLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Purine-cytosine permease FCY2 (FCY2), partial CSB-YP323748SVG
CSB-EP323748SVG
CSB-BP323748SVG
CSB-MP323748SVG
CSB-EP323748SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Urease subunit alpha (ureC), partial CSB-YP323749EYZ
CSB-EP323749EYZ
CSB-BP323749EYZ
CSB-MP323749EYZ
CSB-EP323749EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halobacterium salinarum Uncharacterized protein VNG_1110C (VNG_1110C) CSB-YP323750HTM
CSB-EP323750HTM
CSB-BP323750HTM
CSB-MP323750HTM
CSB-EP323750HTM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Uncharacterized protein J1L CSB-YP323752HWV
CSB-EP323752HWV
CSB-BP323752HWV
CSB-MP323752HWV
CSB-EP323752HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Early phosphoprotein p84 (UL112/UL113), partial CSB-YP323753HWV
CSB-EP323753HWV
CSB-BP323753HWV
CSB-MP323753HWV
CSB-EP323753HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome hibernation promoting factor (hpf) CSB-YP323754DPX
CSB-EP323754DPX
CSB-BP323754DPX
CSB-MP323754DPX
CSB-EP323754DPX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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