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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Phytophthora infestans Actin-1 (ACTA) CSB-YP324335PJO
CSB-EP324335PJO
CSB-BP324335PJO
CSB-MP324335PJO
CSB-EP324335PJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein ATP12, mitochondrial (ATP12), partial CSB-YP324336SVG
CSB-EP324336SVG
CSB-BP324336SVG
CSB-MP324336SVG
CSB-EP324336SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase I subunit RPA135 (RPA135), partial CSB-YP324337SVG
CSB-EP324337SVG
CSB-BP324337SVG
CSB-MP324337SVG
CSB-EP324337SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Proteasome component C11 (PRE1) CSB-YP324338SVG
CSB-EP324338SVG
CSB-BP324338SVG
CSB-MP324338SVG
CSB-EP324338SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 1,3-beta-glucanosyltransferase GAS1 (GAS1), partial CSB-YP324339SVG
CSB-EP324339SVG
CSB-BP324339SVG
CSB-MP324339SVG
CSB-EP324339SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Iron-regulated transcriptional activator AFT1 (AFT1), partial CSB-YP324340SVG
CSB-EP324340SVG
CSB-BP324340SVG
CSB-MP324340SVG
CSB-EP324340SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Nitrate transporter (crnA), partial CSB-YP324341EKF
CSB-EP324341EKF
CSB-BP324341EKF
CSB-MP324341EKF
CSB-EP324341EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable protein E4 (E4) CSB-YP324342HOD
CSB-EP324342HOD
CSB-BP324342HOD
CSB-MP324342HOD
CSB-EP324342HOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhesus papillomavirus type 1 Protein E6 (E6) CSB-YP324343RCO
CSB-EP324343RCO
CSB-BP324343RCO
CSB-MP324343RCO
CSB-EP324343RCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhesus papillomavirus type 1 Major capsid protein L1 (L1), partial CSB-YP324344RCO
CSB-EP324344RCO
CSB-BP324344RCO
CSB-MP324344RCO
CSB-EP324344RCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhesus papillomavirus type 1 Minor capsid protein L2 (L2) CSB-YP324345RCO
CSB-EP324345RCO
CSB-BP324345RCO
CSB-MP324345RCO
CSB-EP324345RCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA replication protein, partial CSB-YP324346FCN
CSB-EP324346FCN
CSB-BP324346FCN
CSB-MP324346FCN
CSB-EP324346FCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coat protein CSB-YP324347FCN
CSB-EP324347FCN
CSB-BP324347FCN
CSB-MP324347FCN
CSB-EP324347FCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Infectious pancreatic necrosis virus RNA-directed RNA polymerase (VP1), partial CSB-YP324348IAV
CSB-EP324348IAV
CSB-BP324348IAV
CSB-MP324348IAV
CSB-EP324348IAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Plasma membrane ATPase 1 (LHA1), partial CSB-YP324349SZY
CSB-EP324349SZY
CSB-BP324349SZY
CSB-MP324349SZY
CSB-EP324349SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein MraZ (mraZ) CSB-YP324350ENV
CSB-EP324350ENV
CSB-BP324350ENV
CSB-MP324350ENV
CSB-EP324350ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized cox1 intron-2 37.2 kDa protein (SPMIT.03) CSB-YP324351SXV
CSB-EP324351SXV
CSB-BP324351SXV
CSB-MP324351SXV
CSB-EP324351SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA damage checkpoint control protein rad1 (rad1) CSB-YP324352SXV
CSB-EP324352SXV
CSB-BP324352SXV
CSB-MP324352SXV
CSB-EP324352SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arachis hypogaea Cationic peroxidase 2 (PNC2) CSB-YP324353ANE
CSB-EP324353ANE
CSB-BP324353ANE
CSB-MP324353ANE
CSB-EP324353ANE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae V-type proton ATPase subunit E (VMA4) CSB-YP324354SVG
CSB-EP324354SVG
CSB-BP324354SVG
CSB-MP324354SVG
CSB-EP324354SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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