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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cucumber mosaic virus Capsid protein (ORF3b) CSB-YP324543CUA
CSB-EP324543CUA
CSB-BP324543CUA
CSB-MP324543CUA
CSB-EP324543CUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sporulation kinase A (kinA), partial CSB-YP324544BRJ
CSB-EP324544BRJ
CSB-BP324544BRJ
CSB-MP324544BRJ
CSB-EP324544BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP324545IFG
CSB-EP324545IFG
CSB-BP324545IFG
CSB-MP324545IFG
CSB-EP324545IFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus phage phi29 Early protein GP16.6 (16.6) CSB-YP324546BBC
CSB-EP324546BBC
CSB-BP324546BBC
CSB-MP324546BBC
CSB-EP324546BBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli DNA replication terminus site-binding protein (tus) CSB-YP324547ENV
CSB-EP324547ENV
CSB-BP324547ENV
CSB-MP324547ENV
CSB-EP324547ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Granaticin polyketide synthase putative ketoacyl reductase 2 (gra-orf6) CSB-YP324548SQH
CSB-EP324548SQH
CSB-BP324548SQH
CSB-MP324548SQH
CSB-EP324548SQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein APA1 (APA1) CSB-YP324549SVG
CSB-EP324549SVG
CSB-BP324549SVG
CSB-MP324549SVG
CSB-EP324549SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multifunctional cyclase-dehydratase-3-O-methyl transferase tcmN (tcmN) CSB-YP324550SOG
CSB-EP324550SOG
CSB-BP324550SOG
CSB-MP324550SOG
CSB-EP324550SOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Allophycocyanin alpha chain 1 (apcA1) CSB-YP324551FCY
CSB-EP324551FCY
CSB-BP324551FCY
CSB-MP324551FCY
CSB-EP324551FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio alginolyticus Alkaline serine exoprotease A (proA) CSB-YP324552VEU
CSB-EP324552VEU
CSB-BP324552VEU
CSB-MP324552VEU
CSB-EP324552VEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rice dwarf virus Suppressor of RNA-mediated gene silencing CSB-YP324553RMI
CSB-EP324553RMI
CSB-BP324553RMI
CSB-MP324553RMI
CSB-EP324553RMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cowpox virus A-type inclusion protein (ATI), partial CSB-YP324554CRF
CSB-EP324554CRF
CSB-BP324554CRF
CSB-MP324554CRF
CSB-EP324554CRF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Measles virus Matrix protein (M) CSB-YP324555MCO
CSB-EP324555MCO
CSB-BP324555MCO
CSB-MP324555MCO
CSB-EP324555MCO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus S Movement protein TGBp3 (ORF4), partial CSB-YP324556PRB
CSB-EP324556PRB
CSB-BP324556PRB
CSB-MP324556PRB
CSB-EP324556PRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP324557ENV
CSB-EP324557ENV
CSB-BP324557ENV
CSB-MP324557ENV
CSB-EP324557ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein phnG (phnG) CSB-YP324558ENV
CSB-EP324558ENV
CSB-BP324558ENV
CSB-MP324558ENV
CSB-EP324558ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein PhnO (phnO) CSB-YP324559ENV
CSB-EP324559ENV
CSB-BP324559ENV
CSB-MP324559ENV
CSB-EP324559ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Autographa californica nuclear polyhedrosis virus Ubiquitin-like protein (V-UBI) CSB-YP324560ARA
CSB-EP324560ARA
CSB-BP324560ARA
CSB-MP324560ARA
CSB-EP324560ARA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Major core protein 4a precursor (VACWR129), partial CSB-YP324561VAI
CSB-EP324561VAI
CSB-BP324561VAI
CSB-MP324561VAI
CSB-EP324561VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Protein UL76 (UL76) CSB-YP324562HWV
CSB-EP324562HWV
CSB-BP324562HWV
CSB-MP324562HWV
CSB-EP324562HWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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