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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP324603FQA
CSB-EP324603FQA
CSB-BP324603FQA
CSB-MP324603FQA
CSB-EP324603FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogen regulatory protein (ptsN) CSB-YP324604KBE
CSB-EP324604KBE
CSB-BP324604KBE
CSB-MP324604KBE
CSB-EP324604KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tryptophan synthase beta chain (trpB) CSB-YP324605LMH
CSB-EP324605LMH
CSB-BP324605LMH
CSB-MP324605LMH
CSB-EP324605LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human cytomegalovirus Envelope glycoprotein H (gH), partial CSB-YP324606HWX
CSB-EP324606HWX
CSB-BP324606HWX
CSB-MP324606HWX
CSB-EP324606HWX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Duck hepatitis B virus External core antigen (C) CSB-YP324607DHF
CSB-EP324607DHF
CSB-BP324607DHF
CSB-MP324607DHF
CSB-EP324607DHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Kinesin heavy chain (Khc), partial CSB-YP324608DLU
CSB-EP324608DLU
CSB-BP324608DLU
CSB-MP324608DLU
CSB-EP324608DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bombyx mori Prothoracicotropic hormone CSB-YP324609BTT
CSB-BP324609BTT
CSB-MP324609BTT
CSB-EP324609BTT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Photosystem I reaction center subunit IX (psaJ), partial CSB-YP324610EWE
CSB-EP324610EWE
CSB-BP324610EWE
CSB-MP324610EWE
CSB-EP324610EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Icaria sp. Chemotactic peptide CSB-YP324611IAB
CSB-EP324611IAB
CSB-BP324611IAB
CSB-MP324611IAB
CSB-EP324611IAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helianthus annuus ATP synthase subunit 9, mitochondrial (ATP9), partial CSB-YP324612HUO1
CSB-EP324612HUO1
CSB-BP324612HUO1
CSB-MP324612HUO1
CSB-EP324612HUO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti RNA polymerase sigma-54 factor (rpoN), partial CSB-YP324613RKU
CSB-EP324613RKU
CSB-BP324613RKU
CSB-MP324613RKU
CSB-EP324613RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila hydei Histone H2B (His2B) CSB-YP324614DLM
CSB-EP324614DLM
CSB-BP324614DLM
CSB-MP324614DLM
CSB-EP324614DLM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mucor circinelloides f. lusitanicus 3-isopropylmalate dehydratase (LEUA), partial CSB-YP324615MIW
CSB-EP324615MIW
CSB-BP324615MIW
CSB-MP324615MIW
CSB-EP324615MIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus Protein Tat (tat) CSB-YP324616SGW
CSB-EP324616SGW
CSB-BP324616SGW
CSB-MP324616SGW
CSB-EP324616SGW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanelle 30S ribosomal protein S12 (rps12) CSB-YP324617DZX
CSB-EP324617DZX
CSB-BP324617DZX
CSB-MP324617DZX
CSB-EP324617DZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase iron protein (nifH) CSB-YP324618DPS
CSB-EP324618DPS
CSB-BP324618DPS
CSB-MP324618DPS
CSB-EP324618DPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Large terminase protein (17), partial CSB-YP324619EDZ
CSB-EP324619EDZ
CSB-BP324619EDZ
CSB-MP324619EDZ
CSB-EP324619EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Artemia franciscana Sodium/potassium-transporting ATPase subunit alpha-A, partial CSB-YP324620DOF
CSB-EP324620DOF
CSB-BP324620DOF
CSB-MP324620DOF
CSB-EP324620DOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica napus Napin (NAP1) CSB-YP324621BWD
CSB-EP324621BWD
CSB-BP324621BWD
CSB-MP324621BWD
CSB-EP324621BWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP324622FKI
CSB-EP324622FKI
CSB-BP324622FKI
CSB-MP324622FKI
CSB-EP324622FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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