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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Plestiodon skiltonianus Protein Wnt-4 (WNT-4) CSB-YP026137PNB
CSB-EP026137PNB
CSB-BP026137PNB
CSB-MP026137PNB
CSB-EP026137PNB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris gallopavo Protein Wnt-5a (WNT5A) CSB-YP026138MQV
CSB-EP026138MQV
CSB-BP026138MQV
CSB-MP026138MQV
CSB-EP026138MQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plethodon jordani Protein Wnt-3a (WNT-3A) CSB-YP026136PNC
CSB-EP026136PNC
CSB-BP026136PNC
CSB-MP026136PNC
CSB-EP026136PNC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plethodon jordani Protein Wnt-7a (WNT-7A) CSB-YP026141PNC
CSB-EP026141PNC
CSB-BP026141PNC
CSB-MP026141PNC
CSB-EP026141PNC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thunnus thynnus Protein Wnt-6 (wnt6) CSB-YP026140TEK
CSB-EP026140TEK
CSB-BP026140TEK
CSB-MP026140TEK
CSB-EP026140TEK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anas platyrhynchos NADP-dependent malic enzyme (ME1), partial CSB-YP013634BZD
CSB-EP013634BZD
CSB-BP013634BZD
CSB-MP013634BZD
CSB-EP013634BZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepomis macrochirus Osteocalcin (bglap) CSB-YP002682LEG
CSB-EP002682LEG
CSB-BP002682LEG
CSB-MP002682LEG
CSB-EP002682LEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serovar 1/2a Superoxide dismutase [Mn] (sodA) CSB-YP022398LPY
CSB-EP022398LPY
CSB-BP022398LPY
CSB-MP022398LPY
CSB-EP022398LPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Ribonucleoside-diphosphate reductase large subunit (21), partial CSB-YP020518EFJ
CSB-EP020518EFJ
CSB-BP020518EFJ
CSB-MP020518EFJ
CSB-EP020518EFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Transcription initiation factor IIB (TfIIB) CSB-YP010001DLU
CSB-EP010001DLU
CSB-BP010001DLU
CSB-MP010001DLU
CSB-EP010001DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome c (CC-1) CSB-YP006328DOA
CSB-EP006328DOA
CSB-BP006328DOA
CSB-MP006328DOA
CSB-EP006328DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fus) CSB-YP009373SPH
CSB-EP009373SPH
CSB-BP009373SPH
CSB-MP009373SPH
CSB-EP009373SPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division control protein 6 homolog (cdc6) CSB-YP005022MEV
CSB-EP005022MEV
CSB-BP005022MEV
CSB-MP005022MEV
CSB-EP005022MEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amblyornis macgregoriae Cytochrome b (MT-CYB), partial CSB-YP015075AIN
CSB-EP015075AIN
CSB-BP015075AIN
CSB-MP015075AIN
CSB-EP015075AIN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pitta sordida Cytochrome b (MT-CYB), partial CSB-YP015075PKY
CSB-EP015075PKY
CSB-BP015075PKY
CSB-MP015075PKY
CSB-EP015075PKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atractosteus spatula Cytochrome c oxidase subunit 1 (mt-co1), partial CSB-YP015072AQQ1
CSB-EP015072AQQ1
CSB-BP015072AQQ1
CSB-MP015072AQQ1
CSB-EP015072AQQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Polypterus sp. Cytochrome c oxidase subunit 1 (mt-co1), partial CSB-YP015072POX1
CSB-EP015072POX1
CSB-BP015072POX1
CSB-MP015072POX1
CSB-EP015072POX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Megalops atlanticus Cytochrome c oxidase subunit 2 (mt-co2), partial CSB-YP015073MDE1
CSB-EP015073MDE1
CSB-BP015073MDE1
CSB-MP015073MDE1
CSB-EP015073MDE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geophagus steindachneri Cytochrome b (mt-cyb), partial CSB-YP015075GBN1
CSB-EP015075GBN1
CSB-BP015075GBN1
CSB-MP015075GBN1
CSB-EP015075GBN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pomoxis nigromaculatus Cytochrome b (mt-cyb), partial CSB-YP015075PPN1
CSB-EP015075PPN1
CSB-BP015075PPN1
CSB-MP015075PPN1
CSB-EP015075PPN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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