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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant ATP synthase subunit b (atpF), partial CSB-YP002358MVN1
CSB-EP002358MVN1
CSB-BP002358MVN1
CSB-MP002358MVN1
CSB-EP002358MVN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit a (atpB), partial CSB-YP015070MVN1
CSB-EP015070MVN1
CSB-BP015070MVN1
CSB-MP015070MVN1
CSB-EP015070MVN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP015197MVN
CSB-EP015197MVN
CSB-BP015197MVN
CSB-MP015197MVN
CSB-EP015197MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii 60S ribosomal protein L31 (RPL31) CSB-YP020227DSJ
CSB-EP020227DSJ
CSB-BP020227DSJ
CSB-MP020227DSJ
CSB-EP020227DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Acetyl-CoA acetyltransferase (mmgA) CSB-YP001134BRJ
CSB-EP001134BRJ
CSB-BP001134BRJ
CSB-MP001134BRJ
CSB-EP001134BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chondroitin sulfate synthase 2 (mig-22), partial CSB-YP005373CXY
CSB-EP005373CXY
CSB-BP005373CXY
CSB-MP005373CXY
CSB-EP005373CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Tubulin beta-4 chain (TUBB4) CSB-YP025324OFG
CSB-EP025324OFG
CSB-BP025324OFG
CSB-MP025324OFG
CSB-EP025324OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mediator of RNA polymerase II transcription subunit 10 (mdt-10) CSB-YP013641CXY
CSB-EP013641CXY
CSB-BP013641CXY
CSB-MP013641CXY
CSB-EP013641CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacter aerogenes DNA topoisomerase 1 (topA) CSB-YP024056EKN
CSB-EP024056EKN
CSB-BP024056EKN
CSB-MP024056EKN
CSB-EP024056EKN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Argininosuccinate synthase (ARG1) CSB-YP002234SVG
CSB-EP002234SVG
CSB-BP002234SVG
CSB-MP002234SVG
CSB-EP002234SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus sp. Aspartate aminotransferase CSB-YP009681BCN
CSB-EP009681BCN
CSB-BP009681BCN
CSB-MP009681BCN
CSB-EP009681BCN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Elongation factor G (fusA) CSB-YP009373GFM
CSB-EP009373GFM
CSB-BP009373GFM
CSB-MP009373GFM
CSB-EP009373GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c1 (petC), partial CSB-YP006327RMA1
CSB-EP006327RMA1
CSB-BP006327RMA1
CSB-MP006327RMA1
CSB-EP006327RMA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Uricase (Uro) CSB-YP025648DMP
CSB-EP025648DMP
CSB-BP025648DMP
CSB-MP025648DMP
CSB-EP025648DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcription initiation factor TFIID subunit 2 (TAF2), partial CSB-YP023088SVG
CSB-EP023088SVG
CSB-BP023088SVG
CSB-MP023088SVG
CSB-EP023088SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Anion exchange protein 2 (Slc4a2), partial CSB-YP021667RA
CSB-EP021667RA
CSB-BP021667RA
CSB-MP021667RA
CSB-EP021667RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster V-type proton ATPase 16 kDa proteolipid subunit (Vha16-1), partial CSB-YP002389DLU1
CSB-EP002389DLU1
CSB-BP002389DLU1
CSB-MP002389DLU1
CSB-EP002389DLU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica SPARC-like protein 1 (SPARCL1), partial CSB-YP022487DXJ
CSB-EP022487DXJ
CSB-BP022487DXJ
CSB-MP022487DXJ
CSB-EP022487DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latimeria chalumnae Hemoglobin subunit beta (HBB) CSB-YP010150LNS
CSB-EP010150LNS
CSB-BP010150LNS
CSB-MP010150LNS
CSB-EP010150LNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Glutamine synthetase (glnA) CSB-YP009553FPM
CSB-EP009553FPM
CSB-BP009553FPM
CSB-MP009553FPM
CSB-EP009553FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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