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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Reithrodontomys megalotis NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078RJZ1
CSB-EP015078RJZ1
CSB-BP015078RJZ1
CSB-MP015078RJZ1
CSB-EP015078RJZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Osgoodomys banderanus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078OFL1
CSB-EP015078OFL1
CSB-BP015078OFL1
CSB-MP015078OFL1
CSB-EP015078OFL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podomys floridanus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078EXH1
CSB-EP015078EXH1
CSB-BP015078EXH1
CSB-MP015078EXH1
CSB-EP015078EXH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ctenomys frater Cytochrome b (MT-CYB), partial CSB-YP015075DYY
CSB-EP015075DYY
CSB-BP015075DYY
CSB-MP015075DYY
CSB-EP015075DYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ctenomys steinbachi Cytochrome b (MT-CYB), partial CSB-YP015075DZD
CSB-EP015075DZD
CSB-BP015075DZD
CSB-MP015075DZD
CSB-EP015075DZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage PST Single-stranded DNA-binding protein (32) CSB-YP022706ELE
CSB-EP022706ELE
CSB-BP022706ELE
CSB-MP022706ELE
CSB-EP022706ELE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage RB15 Single-stranded DNA-binding protein (32) CSB-YP022706ELG
CSB-EP022706ELG
CSB-BP022706ELG
CSB-MP022706ELG
CSB-EP022706ELG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage SV76 Single-stranded DNA-binding protein (32) CSB-YP022706ELR
CSB-EP022706ELR
CSB-BP022706ELR
CSB-MP022706ELR
CSB-EP022706ELR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ubiquitin carboxyl-terminal hydrolase 5 (UBP5), partial CSB-YP025742DOA
CSB-EP025742DOA
CSB-BP025742DOA
CSB-MP025742DOA
CSB-EP025742DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 40S ribosomal protein S4 (RPS4) CSB-YP020458ZAX
CSB-EP020458ZAX
CSB-BP020458ZAX
CSB-MP020458ZAX
CSB-EP020458ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dunaliella salina 60S ribosomal protein L5 (RPL5) CSB-YP020297DMW
CSB-EP020297DMW
CSB-BP020297DMW
CSB-MP020297DMW
CSB-EP020297DMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fritillaria agrestis Cytochrome c (CYTC) CSB-YP006328FFL
CSB-EP006328FFL
CSB-BP006328FFL
CSB-MP006328FFL
CSB-EP006328FFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Formin-like protein 2 (FH2), partial CSB-YP008744DOA
CSB-EP008744DOA
CSB-BP008744DOA
CSB-MP008744DOA
CSB-EP008744DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L36a (RPL36AA) CSB-YP020253DOA
CSB-EP020253DOA
CSB-BP020253DOA
CSB-MP020253DOA
CSB-EP020253DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Formin-like protein 3 (FH3), partial CSB-YP008745DOA1
CSB-EP008745DOA1
CSB-BP008745DOA1
CSB-MP008745DOA1
CSB-EP008745DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana V-type proton ATPase catalytic subunit A (VHA-A), partial CSB-YP002396DOA
CSB-EP002396DOA
CSB-BP002396DOA
CSB-MP002396DOA
CSB-EP002396DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta vulgaris Elongation factor 2, partial CSB-YP007434BSQ
CSB-EP007434BSQ
CSB-BP007434BSQ
CSB-MP007434BSQ
CSB-EP007434BSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Olea europaea Profilin-1 (PRO1) CSB-YP017824OEC
CSB-EP017824OEC
CSB-BP017824OEC
CSB-MP017824OEC
CSB-EP017824OEC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea Proteasome subunit beta type-5 CSB-YP018883FKI
CSB-EP018883FKI
CSB-BP018883FKI
CSB-MP018883FKI
CSB-EP018883FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. tRNA pseudouridine synthase A (truA) CSB-YP019086FPZ
CSB-EP019086FPZ
CSB-BP019086FPZ
CSB-MP019086FPZ
CSB-EP019086FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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