Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus laevis Transcription factor Sox-7 (sox7) CSB-YP022435XBE
CSB-EP022435XBE
CSB-BP022435XBE
CSB-MP022435XBE
CSB-EP022435XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes 40S ribosomal protein S24 (rps24) CSB-YP020402TKY
CSB-EP020402TKY
CSB-BP020402TKY
CSB-MP020402TKY
CSB-EP020402TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua Hemoglobin subunit alpha-2 (hba2) CSB-YP010148GER
CSB-EP010148GER
CSB-BP010148GER
CSB-MP010148GER
CSB-EP010148GER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pygoscelis adelie Thyroid hormone receptor alpha (THRA) CSB-YP023510FHP
CSB-EP023510FHP
CSB-BP023510FHP
CSB-MP023510FHP
CSB-EP023510FHP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetraodon fluviatilis SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 (smarcb1) CSB-YP021805TLV
CSB-EP021805TLV
CSB-BP021805TLV
CSB-MP021805TLV
CSB-EP021805TLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transducin-like enhancer protein 4 (tle4), partial CSB-YP023588XBE
CSB-EP023588XBE
CSB-BP023588XBE
CSB-MP023588XBE
CSB-EP023588XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis ER lumen protein retaining receptor 3 (kdelr3), partial CSB-YP012131XBE1
CSB-EP012131XBE1
CSB-BP012131XBE1
CSB-MP012131XBE1
CSB-EP012131XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pantoea ananatis Ribonuclease 3 (rnc) CSB-YP019809ERC
CSB-EP019809ERC
CSB-BP019809ERC
CSB-MP019809ERC
CSB-EP019809ERC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanohalophilus mahii FAD synthase (ribL) CSB-YP008710MSG
CSB-EP008710MSG
CSB-BP008710MSG
CSB-MP008710MSG
CSB-EP008710MSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tuber melanosporum U1 small nuclear ribonucleoprotein C (GSTUM_00001021001) CSB-YP022331TRB
CSB-EP022331TRB
CSB-BP022331TRB
CSB-MP022331TRB
CSB-EP022331TRB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Probable N-acetyltransferase 8B (Nat8b), partial CSB-YP015475MO1
CSB-EP015475MO1
CSB-BP015475MO1
CSB-MP015475MO1
CSB-EP015475MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pediculus humanus subsp. corporis Inosine triphosphate pyrophosphatase (Phum_PHUM462900) CSB-YP011907EST
CSB-EP011907EST
CSB-BP011907EST
CSB-MP011907EST
CSB-EP011907EST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cyclin-dependent kinase 12 (CDK12), partial CSB-YP005981BO
CSB-EP005981BO
CSB-BP005981BO
CSB-MP005981BO
CSB-EP005981BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Serine/threonine-protein kinase 10 (STK10), partial CSB-YP022832BO
CSB-EP022832BO
CSB-BP022832BO
CSB-MP022832BO
CSB-EP022832BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken U1 small nuclear ribonucleoprotein C (SNRPC) CSB-YP022331CH
CSB-EP022331CH
CSB-BP022331CH
CSB-MP022331CH
CSB-EP022331CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoplanus petrolearius FAD synthase (ribL) CSB-YP008710MSH
CSB-EP008710MSH
CSB-BP008710MSH
CSB-MP008710MSH
CSB-EP008710MSH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dicentrarchus labrax F-box/LRR-repeat protein 15 (fbxl15) CSB-YP008466DKC
CSB-EP008466DKC
CSB-BP008466DKC
CSB-MP008466DKC
CSB-EP008466DKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Nanos homolog 1 (nanos1) CSB-YP015436DIL
CSB-EP015436DIL
CSB-BP015436DIL
CSB-MP015436DIL
CSB-EP015436DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse PR domain zinc finger protein 13 (Prdm13) CSB-YP018642MO
CSB-EP018642MO
CSB-BP018642MO
CSB-MP018642MO
CSB-EP018642MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Misshapen-like kinase 1 (Mink1), partial CSB-YP013830RA
CSB-EP013830RA
CSB-BP013830RA
CSB-MP013830RA
CSB-EP013830RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>