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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Ubiquitin carboxyl-terminal hydrolase 3 (UBP3) CSB-YP025720DOA
CSB-EP025720DOA
CSB-BP025720DOA
CSB-MP025720DOA
CSB-EP025720DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays S-adenosylmethionine decarboxylase proenzyme (SAMDC) CSB-YP001658ZAX
CSB-EP001658ZAX
CSB-BP001658ZAX
CSB-MP001658ZAX
CSB-EP001658ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triphysaria versicolor Asparagine synthetase [glutamine-hydrolyzing] (AS), partial CSB-YP002219TQM
CSB-EP002219TQM
CSB-BP002219TQM
CSB-MP002219TQM
CSB-EP002219TQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717HUV
CSB-EP009717HUV
CSB-BP009717HUV
CSB-MP009717HUV
CSB-EP009717HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Signal recognition particle 19 kDa protein (srp19) CSB-YP022674MSR
CSB-EP022674MSR
CSB-BP022674MSR
CSB-MP022674MSR
CSB-EP022674MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Malate dehydrogenase (mdh) CSB-YP013623MSR
CSB-EP013623MSR
CSB-BP013623MSR
CSB-MP013623MSR
CSB-EP013623MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Elongation factor 2 (fusA), partial CSB-YP007434MSR
CSB-EP007434MSR
CSB-BP007434MSR
CSB-MP007434MSR
CSB-EP007434MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Serine hydroxymethyltransferase (glyA) CSB-YP021274MSR
CSB-EP021274MSR
CSB-BP021274MSR
CSB-MP021274MSR
CSB-EP021274MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Adenosylhomocysteinase (ahcY) CSB-YP001474DOC
CSB-EP001474DOC
CSB-BP001474DOC
CSB-MP001474DOC
CSB-EP001474DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Transcription initiation factor IIB (tfb) CSB-YP010001DOC
CSB-EP010001DOC
CSB-BP010001DOC
CSB-MP010001DOC
CSB-EP010001DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Signal recognition particle 19 kDa protein (srp19) CSB-YP022674DOC
CSB-EP022674DOC
CSB-BP022674DOC
CSB-MP022674DOC
CSB-EP022674DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Isocitrate dehydrogenase [NADP] (icd) CSB-YP010990DOC
CSB-EP010990DOC
CSB-BP010990DOC
CSB-MP010990DOC
CSB-EP010990DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate synthase (thyA) CSB-YP025393BQH
CSB-EP025393BQH
CSB-BP025393BQH
CSB-MP025393BQH
CSB-EP025393BQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Elongation factor Tu (tuf) CSB-YP025342BXE
CSB-EP025342BXE
CSB-BP025342BXE
CSB-MP025342BXE
CSB-EP025342BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glycine cleavage system H protein (gcvH) CSB-YP009335BRJ
CSB-EP009335BRJ
CSB-BP009335BRJ
CSB-MP009335BRJ
CSB-EP009335BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Proline dehydrogenase 1 (fadM) CSB-YP018744BRJ
CSB-EP018744BRJ
CSB-BP018744BRJ
CSB-MP018744BRJ
CSB-EP018744BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-recycling factor (frr) CSB-YP014941MVN
CSB-EP014941MVN
CSB-BP014941MVN
CSB-MP014941MVN
CSB-EP014941MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Electron transfer flavoprotein subunit alpha (etfA) CSB-YP007844MVN
CSB-EP007844MVN
CSB-BP007844MVN
CSB-MP007844MVN
CSB-EP007844MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia felis Methionyl-tRNA formyltransferase (fmt) CSB-YP015150RMT
CSB-EP015150RMT
CSB-BP015150RMT
CSB-MP015150RMT
CSB-EP015150RMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP015150RMW
CSB-EP015150RMW
CSB-BP015150RMW
CSB-MP015150RMW
CSB-EP015150RMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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