Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Elongation factor Ts (tsf) CSB-YP025124MVN
CSB-EP025124MVN
CSB-BP025124MVN
CSB-MP025124MVN
CSB-EP025124MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methionyl-tRNA formyltransferase (fmt) CSB-YP015150RMP
CSB-EP015150RMP
CSB-BP015150RMP
CSB-MP015150RMP
CSB-EP015150RMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. viciae Malate dehydrogenase (mdh) CSB-YP013623RKS
CSB-EP013623RKS
CSB-BP013623RKS
CSB-MP013623RKS
CSB-EP013623RKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris L-threonine 3-dehydrogenase (tdh) CSB-YP023350XAY
CSB-EP023350XAY
CSB-BP023350XAY
CSB-MP023350XAY
CSB-EP023350XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Dihydrolipoyl dehydrogenase (acoL), partial CSB-YP006928BRJ
CSB-EP006928BRJ
CSB-BP006928BRJ
CSB-MP006928BRJ
CSB-EP006928BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Argininosuccinate synthase (argG) CSB-YP002234BRJ
CSB-EP002234BRJ
CSB-BP002234BRJ
CSB-MP002234BRJ
CSB-EP002234BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Methionine aminopeptidase 2 (mapB) CSB-YP013716BRJ
CSB-EP013716BRJ
CSB-BP013716BRJ
CSB-MP013716BRJ
CSB-EP013716BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein C10 (Grcc10) CSB-YP003090MO
CSB-EP003090MO
CSB-BP003090MO
CSB-MP003090MO
CSB-EP003090MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus UTP--glucose-1-phosphate uridylyltransferase (UGP2), partial CSB-YP025569DXU
CSB-EP025569DXU
CSB-BP025569DXU
CSB-MP025569DXU
CSB-EP025569DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/arginine-rich splicing factor 5 (Srsf5) CSB-YP021157MO
CSB-EP021157MO
CSB-BP021157MO
CSB-MP021157MO
CSB-EP021157MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Transient receptor potential cation channel subfamily V member 1 (Trpv1), partial CSB-YP025093RA
CSB-EP025093RA
CSB-BP025093RA
CSB-MP025093RA
CSB-EP025093RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Medium-wave-sensitive opsin 1 (Opn1mw), partial CSB-YP016352RA1
CSB-EP016352RA1
CSB-BP016352RA1
CSB-MP016352RA1
CSB-EP016352RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Micromys minutus Phosphatidylcholine-sterol acyltransferase (LCAT) CSB-YP012783MTV
CSB-EP012783MTV
CSB-BP012783MTV
CSB-MP012783MTV
CSB-EP012783MTV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mus caroli Nuclear autoantigen Sp-100 (Sp100) CSB-YP022439MUU
CSB-EP022439MUU
CSB-BP022439MUU
CSB-MP022439MUU
CSB-EP022439MUU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Peptidyl-prolyl cis-trans isomerase FKBP1A (fkbp1a) CSB-YP008691XBE
CSB-EP008691XBE
CSB-BP008691XBE
CSB-MP008691XBE
CSB-EP008691XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pagrus major Estrogen receptor (esr1), partial CSB-YP007830EQR
CSB-EP007830EQR
CSB-BP007830EQR
CSB-MP007830EQR
CSB-EP007830EQR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein mago nashi homolog (magoh) CSB-YP013364XBE
CSB-EP013364XBE
CSB-BP013364XBE
CSB-MP013364XBE
CSB-EP013364XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Neurogenic differentiation factor 1 (neurod1) CSB-YP015725DIL
CSB-EP015725DIL
CSB-BP015725DIL
CSB-MP015725DIL
CSB-EP015725DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oreochromis niloticus Guanine nucleotide-binding protein subunit beta-2-like 1 (gnb2l1) CSB-YP009605OET
CSB-EP009605OET
CSB-BP009605OET
CSB-MP009605OET
CSB-EP009605OET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Electrophorus electricus Acetylcholinesterase (ache), partial CSB-YP001154EJY
CSB-EP001154EJY
CSB-BP001154EJY
CSB-MP001154EJY
CSB-EP001154EJY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>