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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Schizosaccharomyces pombe RNA polymerase II subunit A C-terminal domain phosphatase ssu72 (ssu72) CSB-YP022729SXV
CSB-EP022729SXV
CSB-BP022729SXV
CSB-MP022729SXV
CSB-EP022729SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Exosome complex component rrp46 (rrp46) CSB-YP007893SXV
CSB-EP007893SXV
CSB-BP007893SXV
CSB-MP007893SXV
CSB-EP007893SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Anaphase-promoting complex subunit 10 (apc10) CSB-YP001695SXV
CSB-EP001695SXV
CSB-BP001695SXV
CSB-MP001695SXV
CSB-EP001695SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe U6 snRNA-associated Sm-like protein LSm5 (lsm5) CSB-YP013208SXV
CSB-EP013208SXV
CSB-BP013208SXV
CSB-MP013208SXV
CSB-EP013208SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerase I subunit rpa43 (rpa43) CSB-YP025360SXV
CSB-EP025360SXV
CSB-BP025360SXV
CSB-MP025360SXV
CSB-EP025360SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Decaprenyl-diphosphate synthase subunit 1 (dps1) CSB-YP017744SXV
CSB-EP017744SXV
CSB-BP017744SXV
CSB-MP017744SXV
CSB-EP017744SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S6 (RPS6) CSB-YP020463LOF
CSB-EP020463LOF
CSB-BP020463LOF
CSB-MP020463LOF
CSB-EP020463LOF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila subobscura Uricase (Uro) CSB-YP025648DML
CSB-EP025648DML
CSB-BP025648DML
CSB-MP025648DML
CSB-EP025648DML-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Small nuclear ribonucleoprotein Sm D3 (SmD3) CSB-YP022335DLU
CSB-EP022335DLU
CSB-BP022335DLU
CSB-MP022335DLU
CSB-EP022335DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenylyltransferase and sulfurtransferase MOCS3 (uba-4) CSB-YP014707CXY
CSB-EP014707CXY
CSB-BP014707CXY
CSB-MP014707CXY
CSB-EP014707CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant BAG family molecular chaperone regulator 1 (bag-1) CSB-YP002529CXY
CSB-EP002529CXY
CSB-BP002529CXY
CSB-MP002529CXY
CSB-EP002529CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ADP-ribosylation factor-like protein 3 (arl-3) CSB-YP002084CXY
CSB-EP002084CXY
CSB-BP002084CXY
CSB-MP002084CXY
CSB-EP002084CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caenorhabditis elegans Acid ceramidase (asah-1) CSB-YP002169CXY
CSB-EP002169CXY
CSB-BP002169CXY
CSB-MP002169CXY
CSB-EP002169CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L18 (rpl-18) CSB-YP020154CXY
CSB-EP020154CXY
CSB-BP020154CXY
CSB-MP020154CXY
CSB-EP020154CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lumbricus rubellus 60S ribosomal protein L14 (RPL14) CSB-YP020142LRJ
CSB-EP020142LRJ
CSB-BP020142LRJ
CSB-MP020142LRJ
CSB-EP020142LRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bison bison Sodium/potassium/calcium exchanger 1 (SLC24A1), partial CSB-YP021469BTE1
CSB-EP021469BTE1
CSB-BP021469BTE1
CSB-MP021469BTE1
CSB-EP021469BTE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aotus trivirgatus Ribonuclease K6 (RNASE6) CSB-YP019797DNI
CSB-EP019797DNI
CSB-BP019797DNI
CSB-MP019797DNI
CSB-EP019797DNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saguinus oedipus Ribonuclease K6 (RNASE6) CSB-YP019797SWB
CSB-EP019797SWB
CSB-BP019797SWB
CSB-MP019797SWB
CSB-EP019797SWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Chymotrypsin-like elastase family member 1 (CELA1) CSB-YP007586MOV
CSB-EP007586MOV
CSB-BP007586MOV
CSB-MP007586MOV
CSB-EP007586MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sylvilagus floridanus Cytochrome b (MT-CYB), partial CSB-YP015075FPT
CSB-EP015075FPT
CSB-BP015075FPT
CSB-MP015075FPT
CSB-EP015075FPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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