Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Candida albicans Tubulin alpha chain (TUB1) CSB-YP310429CZF
CSB-EP310429CZF
CSB-BP310429CZF
CSB-MP310429CZF
CSB-EP310429CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe UPF0202 protein C20G8.09c (SPAC20G8.09c), partial CSB-YP310430SXV
CSB-EP310430SXV
CSB-BP310430SXV
CSB-MP310430SXV
CSB-EP310430SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerase II subunit RPB11 (rpb11) CSB-YP310431SXV
CSB-EP310431SXV
CSB-BP310431SXV
CSB-MP310431SXV
CSB-EP310431SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C167.05 (SPAC167.05, SPAC57A7.01), partial CSB-YP310432SXV
CSB-EP310432SXV
CSB-BP310432SXV
CSB-MP310432SXV
CSB-EP310432SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized RING finger protein C57A7.09 (SPAC57A7.09), partial CSB-YP310433SXV1
CSB-EP310433SXV1
CSB-BP310433SXV1
CSB-MP310433SXV1
CSB-EP310433SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mitochondrial inner membrane magnesium transporter mrs2 (mrs2), partial CSB-YP310434SXV1
CSB-EP310434SXV1
CSB-BP310434SXV1
CSB-MP310434SXV1
CSB-EP310434SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable ADP-ribosylation factor-binding protein C25H2.16c (SPBC25H2.16c), partial CSB-YP310435SXV
CSB-EP310435SXV
CSB-BP310435SXV
CSB-MP310435SXV
CSB-EP310435SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable ribonuclease Z 2 (SPBC3D6.03c), partial CSB-YP310436SXV
CSB-EP310436SXV
CSB-BP310436SXV
CSB-MP310436SXV
CSB-EP310436SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe E3 ubiquitin-protein ligase complex slx8-rfp subunit slx8 (slx8) CSB-YP310437SXV
CSB-EP310437SXV
CSB-BP310437SXV
CSB-MP310437SXV
CSB-EP310437SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Alkaline protease 2 (alp2) CSB-YP310438NGS
CSB-BP310438NGS
CSB-MP310438NGS
CSB-EP310438NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Peroxisomal membrane protein PEX17 (PEX17), partial CSB-YP310439YAP
CSB-EP310439YAP
CSB-BP310439YAP
CSB-MP310439YAP
CSB-EP310439YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Sorbose reductase homolog SOU2 (SOU2) CSB-YP310440CZD
CSB-EP310440CZD
CSB-BP310440CZD
CSB-MP310440CZD
CSB-EP310440CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Putative D-3-phosphoglycerate dehydrogenase (SPCC364.07, SPCC4G3.01) CSB-YP310441SXV
CSB-EP310441SXV
CSB-BP310441SXV
CSB-MP310441SXV
CSB-EP310441SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Meiotically up-regulated gene 154 protein (mug154), partial CSB-YP310442SXV1
CSB-EP310442SXV1
CSB-BP310442SXV1
CSB-MP310442SXV1
CSB-EP310442SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Spindle pole body component alp16 (alp16), partial CSB-YP310443SXV
CSB-EP310443SXV
CSB-BP310443SXV
CSB-MP310443SXV
CSB-EP310443SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Protein kinase C-like (NCU06544), partial CSB-YP310444NHA
CSB-EP310444NHA
CSB-BP310444NHA
CSB-MP310444NHA
CSB-EP310444NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR442W (YDR442W), partial CSB-YP310445SVG1
CSB-EP310445SVG1
CSB-BP310445SVG1
CSB-MP310445SVG1
CSB-EP310445SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 11 (AIM11), partial CSB-YP310446SVG1
CSB-EP310446SVG1
CSB-BP310446SVG1
CSB-MP310446SVG1
CSB-EP310446SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein YDR355C (YDR355C), partial CSB-YP310447SVG1
CSB-EP310447SVG1
CSB-BP310447SVG1
CSB-MP310447SVG1
CSB-EP310447SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Pep5-like zinc finger protein C16A10.03c (SPAC16A10.03c), partial CSB-YP310448SXV
CSB-EP310448SXV
CSB-BP310448SXV
CSB-MP310448SXV
CSB-EP310448SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>