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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg01260 (AtMg01260), partial CSB-YP311277DOA1
CSB-EP311277DOA1
CSB-BP311277DOA1
CSB-MP311277DOA1
CSB-EP311277DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg01370 (AtMg01370), partial CSB-YP311278DOA1
CSB-EP311278DOA1
CSB-BP311278DOA1
CSB-MP311278DOA1
CSB-EP311278DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ADP,ATP carrier protein 2, chloroplastic (AATP2), partial CSB-YP311279DOA
CSB-EP311279DOA
CSB-BP311279DOA
CSB-MP311279DOA
CSB-EP311279DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chloride channel protein CLC-b (CLC-B), partial CSB-YP311280DOA
CSB-EP311280DOA
CSB-BP311280DOA
CSB-MP311280DOA
CSB-EP311280DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Arginine/serine-rich-splicing factor RSP40 (RSP40) CSB-YP311281DOA
CSB-EP311281DOA
CSB-BP311281DOA
CSB-MP311281DOA
CSB-EP311281DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Proline dehydrogenase 1, mitochondrial (POX1) CSB-YP311282DOA
CSB-EP311282DOA
CSB-BP311282DOA
CSB-MP311282DOA
CSB-EP311282DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Germin-like protein subfamily 1 member 18 (GLP2A) CSB-YP311283DOA
CSB-EP311283DOA
CSB-BP311283DOA
CSB-MP311283DOA
CSB-EP311283DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 21 (RH21), partial CSB-YP311284DOA
CSB-EP311284DOA
CSB-BP311284DOA
CSB-MP311284DOA
CSB-EP311284DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Vacuolar-sorting receptor 1 (VSR1), partial CSB-YP311285DOA
CSB-EP311285DOA
CSB-BP311285DOA
CSB-MP311285DOA
CSB-EP311285DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sugar transporter ERD6-like 7 (At2g48020), partial CSB-YP311286DOA
CSB-EP311286DOA
CSB-BP311286DOA
CSB-MP311286DOA
CSB-EP311286DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chenopodium rubrum Cell division control protein 2 homolog (CDC2) CSB-YP311287DRT
CSB-EP311287DRT
CSB-BP311287DRT
CSB-MP311287DRT
CSB-EP311287DRT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumis sativus Glutamyl-tRNA reductase 1, chloroplastic (HEMA1) CSB-YP311288CXP
CSB-EP311288CXP
CSB-BP311288CXP
CSB-MP311288CXP
CSB-EP311288CXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Guanine nucleotide-binding protein alpha-2 subunit (GPA2) CSB-YP311289GGV
CSB-EP311289GGV
CSB-BP311289GGV
CSB-MP311289GGV
CSB-EP311289GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ipomoea nil Receptor-like protein kinase (INRPK1), partial CSB-YP311290INC
CSB-EP311290INC
CSB-BP311290INC
CSB-MP311290INC
CSB-EP311290INC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum 14-3-3 protein 8 (TFT8) CSB-YP311291SZY
CSB-EP311291SZY
CSB-BP311291SZY
CSB-MP311291SZY
CSB-EP311291SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesembryanthemum crystallinum 14-3-3-like protein (GBF) CSB-YP311292MRC
CSB-EP311292MRC
CSB-BP311292MRC
CSB-MP311292MRC
CSB-EP311292MRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00130 (AtMg00130) CSB-YP311293DOA
CSB-EP311293DOA
CSB-BP311293DOA
CSB-MP311293DOA
CSB-EP311293DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized mitochondrial protein AtMg00260 (AtMg00260) CSB-YP311294DOA
CSB-EP311294DOA
CSB-BP311294DOA
CSB-MP311294DOA
CSB-EP311294DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ATP synthase subunit a-1 (ATP6-1), partial CSB-YP311295DOA1
CSB-EP311295DOA1
CSB-BP311295DOA1
CSB-MP311295DOA1
CSB-EP311295DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ATP synthase protein YMF19 (YMF19), partial CSB-YP311296DOA
CSB-EP311296DOA
CSB-BP311296DOA
CSB-MP311296DOA
CSB-EP311296DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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