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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Uncharacterized protein YPL068C (YPL068C) CSB-YP311621SVG
CSB-EP311621SVG
CSB-BP311621SVG
CSB-MP311621SVG
CSB-EP311621SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial protein PET191 (PET191) CSB-YP311622SVG
CSB-EP311622SVG
CSB-BP311622SVG
CSB-MP311622SVG
CSB-EP311622SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial inner membrane magnesium transporter MFM1 (MFM1), partial CSB-YP311623SVG1
CSB-EP311623SVG1
CSB-BP311623SVG1
CSB-MP311623SVG1
CSB-EP311623SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP311624SIU
CSB-EP311624SIU
CSB-BP311624SIU
CSB-MP311624SIU
CSB-EP311624SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ornithine decarboxylase antizyme (OAZ1) CSB-YP311625SVG
CSB-EP311625SVG
CSB-BP311625SVG
CSB-MP311625SVG
CSB-EP311625SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus agm.grivet Envelope glycoprotein gp160 (env), partial CSB-YP311626SHF
CSB-EP311626SHF
CSB-BP311626SHF
CSB-MP311626SHF
CSB-EP311626SHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Simian immunodeficiency virus agm.grivet Gag polyprotein (gag) CSB-YP311627SHF
CSB-EP311627SHF
CSB-BP311627SHF
CSB-MP311627SHF
CSB-EP311627SHF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Ts, chloroplastic (tsf) CSB-YP311628ALT
CSB-EP311628ALT
CSB-BP311628ALT
CSB-MP311628ALT
CSB-EP311628ALT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrial (FMP30), partial CSB-YP311629SVG1
CSB-EP311629SVG1
CSB-BP311629SVG1
CSB-MP311629SVG1
CSB-EP311629SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Autophagy-related protein 21 (ATG21) CSB-YP311630SVG
CSB-EP311630SVG
CSB-BP311630SVG
CSB-MP311630SVG
CSB-EP311630SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Glutelin type-B 2 (GLUB2) CSB-YP311631OFG
CSB-EP311631OFG
CSB-BP311631OFG
CSB-MP311631OFG
CSB-EP311631OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Phosphomethylpyrimidine synthase (thiC) CSB-YP311632SIU
CSB-EP311632SIU
CSB-BP311632SIU
CSB-MP311632SIU
CSB-EP311632SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine max Early nodulin-55-2 (ENOD55-2) CSB-YP311633GGV
CSB-EP311633GGV
CSB-BP311633GGV
CSB-MP311633GGV
CSB-EP311633GGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Allophycocyanin alpha chain (apcA) CSB-YP311634SSO
CSB-EP311634SSO
CSB-BP311634SSO
CSB-MP311634SSO
CSB-EP311634SSO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae NET1-associated nuclear protein 1 (NAN1), partial CSB-YP311635SVG
CSB-EP311635SVG
CSB-BP311635SVG
CSB-MP311635SVG
CSB-EP311635SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cauliflower mosaic virus Capsid protein (ORF IV) CSB-YP311636CEZ
CSB-EP311636CEZ
CSB-BP311636CEZ
CSB-MP311636CEZ
CSB-EP311636CEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Keratin, glycine/tyrosine-rich of hair (KAP6-1) CSB-YP311637SH
CSB-EP311637SH
CSB-BP311637SH
CSB-MP311637SH
CSB-EP311637SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Peroxisomal membrane protein PEX25 (PEX25), partial CSB-YP311638SVG
CSB-EP311638SVG
CSB-BP311638SVG
CSB-MP311638SVG
CSB-EP311638SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cochliobolus heterostrophus Mating-type protein MAT-1 (MAT1) CSB-YP311639DUZ
CSB-EP311639DUZ
CSB-BP311639DUZ
CSB-MP311639DUZ
CSB-EP311639DUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solibacter usitatus Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP311640SIU
CSB-EP311640SIU
CSB-BP311640SIU
CSB-MP311640SIU
CSB-EP311640SIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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