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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pinctada maxima EGF-like domain-containing protein 1 CSB-YP311217PYQ
CSB-EP311217PYQ
CSB-BP311217PYQ
CSB-MP311217PYQ
CSB-EP311217PYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinctada maxima Glycine and tyrosine-rich protein CSB-YP311218PYQ
CSB-EP311218PYQ
CSB-BP311218PYQ
CSB-MP311218PYQ
CSB-EP311218PYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinctada maxima Glycine-rich protein 2 CSB-YP311219PYQ
CSB-EP311219PYQ
CSB-BP311219PYQ
CSB-MP311219PYQ
CSB-EP311219PYQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podarcis sicula Tissue- and phase-specific nuclear protein CSB-YP311220PNR
CSB-EP311220PNR
CSB-BP311220PNR
CSB-MP311220PNR
CSB-EP311220PNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhopalurus junceus Non-toxic venom protein CSB-YP311221RMG
CSB-EP311221RMG
CSB-BP311221RMG
CSB-MP311221RMG
CSB-EP311221RMG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Ras-like protein (ras1) CSB-YP311222BVC
CSB-EP311222BVC
CSB-BP311222BVC
CSB-MP311222BVC
CSB-EP311222BVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ustilago maydis Guanine nucleotide-binding protein alpha-3 subunit (GPA3) CSB-YP311223UBS
CSB-EP311223UBS
CSB-BP311223UBS
CSB-MP311223UBS
CSB-EP311223UBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe G1/S-specific cyclin pas1 (pas1) CSB-YP311224SXV
CSB-EP311224SXV
CSB-BP311224SXV
CSB-MP311224SXV
CSB-EP311224SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Non-histone chromosomal protein 6 (nhp6) CSB-YP311225SXV
CSB-EP311225SXV
CSB-BP311225SXV
CSB-MP311225SXV
CSB-EP311225SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chitin synthase 1 (chs1), partial CSB-YP311226PIL
CSB-EP311226PIL
CSB-BP311226PIL
CSB-MP311226PIL
CSB-EP311226PIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Myo-inositol transporter 2 (itr2), partial CSB-YP311227SXV
CSB-EP311227SXV
CSB-BP311227SXV
CSB-MP311227SXV
CSB-EP311227SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable ribonuclease P protein subunit 3 (SPAC3A12.04c) CSB-YP311228SXV
CSB-EP311228SXV
CSB-BP311228SXV
CSB-MP311228SXV
CSB-EP311228SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Protein atp11, mitochondrial (atp11), partial CSB-YP311229SXV
CSB-EP311229SXV
CSB-BP311229SXV
CSB-MP311229SXV
CSB-EP311229SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C57A7.06 (SPAC57A7.06), partial CSB-YP311230SXV
CSB-EP311230SXV
CSB-BP311230SXV
CSB-MP311230SXV
CSB-EP311230SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C25H2.10c (SPBC25H2.10c) CSB-YP311231SXV
CSB-EP311231SXV
CSB-BP311231SXV
CSB-MP311231SXV
CSB-EP311231SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uba3-binding protein but2 (but2) CSB-YP311232SXV
CSB-EP311232SXV
CSB-BP311232SXV
CSB-MP311232SXV
CSB-EP311232SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA polymerase epsilon subunit D (dpb4) CSB-YP311233SXV
CSB-EP311233SXV
CSB-BP311233SXV
CSB-MP311233SXV
CSB-EP311233SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Cell wall integrity and stress response component 1 (wsc1), partial CSB-YP311234SXV1
CSB-EP311234SXV1
CSB-BP311234SXV1
CSB-MP311234SXV1
CSB-EP311234SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Probable mannosyltransferase MNT3 (MNT3), partial CSB-YP311235CZD
CSB-EP311235CZD
CSB-BP311235CZD
CSB-MP311235CZD
CSB-EP311235CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Heat shock protein SSB1 (SSB1), partial CSB-YP311236CZE
CSB-EP311236CZE
CSB-BP311236CZE
CSB-MP311236CZE
CSB-EP311236CZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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