Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Probable propionyl-CoA carboxylase beta chain 5 (accD5), partial CSB-YP311418MVZ
CSB-EP311418MVZ
CSB-BP311418MVZ
CSB-MP311418MVZ
CSB-EP311418MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemoglobin-haptoglobin utilization protein B (hpuB), partial CSB-YP311419NGI
CSB-EP311419NGI
CSB-BP311419NGI
CSB-MP311419NGI
CSB-EP311419NGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Aliphatic sulfonates import ATP-binding protein SsuB (ssuB) CSB-YP311420BRJ
CSB-EP311420BRJ
CSB-BP311420BRJ
CSB-MP311420BRJ
CSB-EP311420BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai tRNA modification GTPase MnmE (mnmE) CSB-YP311421LOS
CSB-EP311421LOS
CSB-BP311421LOS
CSB-MP311421LOS
CSB-EP311421LOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Threonine-phosphate decarboxylase (cobD) CSB-YP311422SXB
CSB-EP311422SXB
CSB-BP311422SXB
CSB-MP311422SXB
CSB-EP311422SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori CAG pathogenicity island protein 13 (cagS) CSB-YP311423HUV
CSB-EP311423HUV
CSB-BP311423HUV
CSB-MP311423HUV
CSB-EP311423HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Mast cell protease 4 (Mcpt4) CSB-YP311424RA
CSB-BP311424RA
CSB-MP311424RA
CSB-EP311424RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas stutzeri Nitric oxide reductase subunit B (norB), partial CSB-YP311425FGJ
CSB-EP311425FGJ
CSB-BP311425FGJ
CSB-MP311425FGJ
CSB-EP311425FGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desmodus rotundus Salivary plasminogen activator alpha 1 CSB-YP311426DCY
CSB-EP311426DCY
CSB-BP311426DCY
CSB-MP311426DCY
CSB-EP311426DCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Tubulin beta-5 chain (Tubb5) CSB-YP311427MO
CSB-BP311427MO
CSB-MP311427MO
CSB-EP311427MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Unknown protein from spot 2D-0014M9 of 2D-PAGE of liver tissue CSB-YP311428MO
CSB-EP311428MO
CSB-BP311428MO
CSB-MP311428MO
CSB-EP311428MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 33 kDa chaperonin (hslO) CSB-YP311429SKY
CSB-EP311429SKY
CSB-BP311429SKY
CSB-MP311429SKY
CSB-EP311429SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Non-canonical purine NTP pyrophosphatase (SA0998) CSB-YP311430SKY
CSB-EP311430SKY
CSB-BP311430SKY
CSB-MP311430SKY
CSB-EP311430SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 30S ribosomal protein S6 (rpsF) CSB-YP311431SKY
CSB-EP311431SKY
CSB-BP311431SKY
CSB-MP311431SKY
CSB-EP311431SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Alanine dehydrogenase 1 (ald1) CSB-YP311432SKY
CSB-EP311432SKY
CSB-BP311432SKY
CSB-MP311432SKY
CSB-EP311432SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP311433SKY
CSB-EP311433SKY
CSB-BP311433SKY
CSB-MP311433SKY
CSB-EP311433SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barley yellow dwarf virus Major capsid protein (ORF3) CSB-YP311434BEO
CSB-EP311434BEO
CSB-BP311434BEO
CSB-MP311434BEO
CSB-EP311434BEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant rRNA adenine N-6-methyltransferase (ermGT) CSB-YP311435LBA
CSB-EP311435LBA
CSB-BP311435LBA
CSB-MP311435LBA
CSB-EP311435LBA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase for transposon gamma-delta (tnpA), partial CSB-YP311437ENV
CSB-EP311437ENV
CSB-BP311437ENV
CSB-MP311437ENV
CSB-EP311437ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Asp-hemolysin (AFUA_3G00590) CSB-YP311438NGS
CSB-EP311438NGS
CSB-BP311438NGS
CSB-MP311438NGS
CSB-EP311438NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>