Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein ydbC (ydbC) CSB-YP311016BRJ
CSB-EP311016BRJ
CSB-BP311016BRJ
CSB-MP311016BRJ
CSB-EP311016BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis C4-dicarboxylate transport protein (dctA), partial CSB-YP311017BRJ
CSB-EP311017BRJ
CSB-BP311017BRJ
CSB-MP311017BRJ
CSB-EP311017BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative acyl-CoA dehydrogenase YdbM (ydbM) CSB-YP311018BRJ
CSB-EP311018BRJ
CSB-BP311018BRJ
CSB-MP311018BRJ
CSB-EP311018BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0699 transmembrane protein ydbT (ydbT), partial CSB-YP311019BRJ1
CSB-EP311019BRJ1
CSB-BP311019BRJ1
CSB-MP311019BRJ1
CSB-EP311019BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydcH (ydcH) CSB-YP311020BRJ
CSB-EP311020BRJ
CSB-BP311020BRJ
CSB-MP311020BRJ
CSB-EP311020BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative DNA relaxase nicK (nicK) CSB-YP311021BRJ
CSB-EP311021BRJ
CSB-BP311021BRJ
CSB-MP311021BRJ
CSB-EP311021BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yddD (yddD), partial CSB-YP311022BRJ1
CSB-EP311022BRJ1
CSB-BP311022BRJ1
CSB-MP311022BRJ1
CSB-EP311022BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Response regulator aspartate phosphatase I (rapI) CSB-YP311023BRJ
CSB-EP311023BRJ
CSB-BP311023BRJ
CSB-MP311023BRJ
CSB-EP311023BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator lrpA (lrpA) CSB-YP311024BRJ
CSB-EP311024BRJ
CSB-BP311024BRJ
CSB-MP311024BRJ
CSB-EP311024BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized transporter ydeD (ydeD), partial CSB-YP311025BRJ
CSB-EP311025BRJ
CSB-BP311025BRJ
CSB-MP311025BRJ
CSB-EP311025BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative hydrolase ydeN (ydeN) CSB-YP311026BRJ
CSB-EP311026BRJ
CSB-BP311026BRJ
CSB-MP311026BRJ
CSB-EP311026BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydfE (ydfE) CSB-YP311027BRJ
CSB-EP311027BRJ
CSB-BP311027BRJ
CSB-MP311027BRJ
CSB-EP311027BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Membrane protein ydfJ (ydfJ), partial CSB-YP311028BRJ
CSB-EP311028BRJ
CSB-BP311028BRJ
CSB-MP311028BRJ
CSB-EP311028BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0702 transmembrane protein ydfS (ydfS), partial CSB-YP311029BRJ1
CSB-EP311029BRJ1
CSB-BP311029BRJ1
CSB-MP311029BRJ1
CSB-EP311029BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydgC (ydgC) CSB-YP311030BRJ
CSB-EP311030BRJ
CSB-BP311030BRJ
CSB-MP311030BRJ
CSB-EP311030BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Arabinose metabolism transcriptional repressor (araR) CSB-YP311031BRJ
CSB-EP311031BRJ
CSB-BP311031BRJ
CSB-MP311031BRJ
CSB-EP311031BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywqI (ywqI) CSB-YP311032BRJ
CSB-EP311032BRJ
CSB-BP311032BRJ
CSB-MP311032BRJ
CSB-EP311032BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative NAD (P)H-dependent FMN-containing oxidoreductase ywqN CSB-YP311033BRJ
CSB-EP311033BRJ
CSB-BP311033BRJ
CSB-MP311033BRJ
CSB-EP311033BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein YwtE (ywtE) CSB-YP311034BRJ
CSB-EP311034BRJ
CSB-BP311034BRJ
CSB-MP311034BRJ
CSB-EP311034BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium pseudotuberculosis 3-dehydroquinate dehydratase (aroQ) CSB-YP311035CQN
CSB-EP311035CQN
CSB-BP311035CQN
CSB-MP311035CQN
CSB-EP311035CQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>