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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pseudomonas aeruginosa Regulatory protein recX (recX) CSB-YP311681PUC
CSB-EP311681PUC
CSB-BP311681PUC
CSB-MP311681PUC
CSB-EP311681PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP311682PUC
CSB-EP311682PUC
CSB-BP311682PUC
CSB-MP311682PUC
CSB-EP311682PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP311683PUC
CSB-EP311683PUC
CSB-BP311683PUC
CSB-MP311683PUC
CSB-EP311683PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Lipid-A-disaccharide synthase (lpxB) CSB-YP311684PUC
CSB-EP311684PUC
CSB-BP311684PUC
CSB-MP311684PUC
CSB-EP311684PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Uridylate kinase (pyrH) CSB-YP311685PUC
CSB-EP311685PUC
CSB-BP311685PUC
CSB-MP311685PUC
CSB-EP311685PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Adenylate kinase (adk) CSB-YP311686PUC
CSB-EP311686PUC
CSB-BP311686PUC
CSB-MP311686PUC
CSB-EP311686PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP311687PUC
CSB-EP311687PUC
CSB-BP311687PUC
CSB-MP311687PUC
CSB-EP311687PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Chaperone protein hscA homolog (hscA), partial CSB-YP311688PUC
CSB-EP311688PUC
CSB-BP311688PUC
CSB-MP311688PUC
CSB-EP311688PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP311689PUC
CSB-EP311689PUC
CSB-BP311689PUC
CSB-MP311689PUC
CSB-EP311689PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP311690PUC
CSB-EP311690PUC
CSB-BP311690PUC
CSB-MP311690PUC
CSB-EP311690PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP311691PUC
CSB-EP311691PUC
CSB-BP311691PUC
CSB-MP311691PUC
CSB-EP311691PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Tyrosine--tRNA ligase (tyrS) CSB-YP311692PUC
CSB-EP311692PUC
CSB-BP311692PUC
CSB-MP311692PUC
CSB-EP311692PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L15 (rplO) CSB-YP311693PUC
CSB-EP311693PUC
CSB-BP311693PUC
CSB-MP311693PUC
CSB-EP311693PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 50S ribosomal protein L2 (rplB) CSB-YP311694PUC
CSB-EP311694PUC
CSB-BP311694PUC
CSB-MP311694PUC
CSB-EP311694PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP311695PUC
CSB-EP311695PUC
CSB-BP311695PUC
CSB-MP311695PUC
CSB-EP311695PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Type III pantothenate kinase (coaX) CSB-YP311696PUC
CSB-EP311696PUC
CSB-BP311696PUC
CSB-MP311696PUC
CSB-EP311696PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (coq7) CSB-YP311697PUC
CSB-EP311697PUC
CSB-BP311697PUC
CSB-MP311697PUC
CSB-EP311697PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa 30S ribosomal protein S21 (rpsU) CSB-YP311698PUC
CSB-EP311698PUC
CSB-BP311698PUC
CSB-MP311698PUC
CSB-EP311698PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoglycerate kinase (pgk) CSB-YP311699PUC
CSB-EP311699PUC
CSB-BP311699PUC
CSB-MP311699PUC
CSB-EP311699PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa UPF0301 protein PA14_05290 (PA14_05290) CSB-YP311700PUC
CSB-EP311700PUC
CSB-BP311700PUC
CSB-MP311700PUC
CSB-EP311700PUC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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