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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Emericella nidulans Putative sterigmatocystin biosynthesis monooxygenase stcW (stcW), partial CSB-YP311480EKF
CSB-EP311480EKF
CSB-BP311480EKF
CSB-MP311480EKF
CSB-EP311480EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Relaxosome protein TraY (traY) CSB-YP311481ENL
CSB-EP311481ENL
CSB-BP311481ENL
CSB-MP311481ENL
CSB-EP311481ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multiple sugar-binding protein (msmE) CSB-YP311482FMR
CSB-EP311482FMR
CSB-BP311482FMR
CSB-MP311482FMR
CSB-EP311482FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Multiple sugar-binding transport system permease protein msmG (msmG), partial CSB-YP311483FMR1
CSB-EP311483FMR1
CSB-BP311483FMR1
CSB-MP311483FMR1
CSB-EP311483FMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Stage V sporulation protein B (spoVB), partial CSB-YP311484BRJ
CSB-EP311484BRJ
CSB-BP311484BRJ
CSB-MP311484BRJ
CSB-EP311484BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin 1 (groL1), partial CSB-YP311485FNU
CSB-EP311485FNU
CSB-BP311485FNU
CSB-MP311485FNU
CSB-EP311485FNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitogen-activated protein kinase SLT2/MPK1 (SLT2) CSB-YP311486SVG
CSB-EP311486SVG
CSB-BP311486SVG
CSB-MP311486SVG
CSB-EP311486SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 6.1 kDa protein in uvsW-uvsY intergenic region (y11A) CSB-YP311487EDZ
CSB-EP311487EDZ
CSB-BP311487EDZ
CSB-MP311487EDZ
CSB-EP311487EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Quinate repressor protein (qutR), partial CSB-YP311488EKF
CSB-EP311488EKF
CSB-BP311488EKF
CSB-MP311488EKF
CSB-EP311488EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui DNA-directed RNA polymerase subunit D (rpoD) CSB-YP311489HTJ
CSB-EP311489HTJ
CSB-BP311489HTJ
CSB-MP311489HTJ
CSB-EP311489HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Odontella sinensis ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP311490OAK1
CSB-EP311490OAK1
CSB-BP311490OAK1
CSB-MP311490OAK1
CSB-EP311490OAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strawberry mild yellow edge-associated virus Movement and silencing protein TGBp1 (ORF2) CSB-YP311491SMC
CSB-EP311491SMC
CSB-BP311491SMC
CSB-MP311491SMC
CSB-EP311491SMC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloarcula marismortui 30S ribosomal protein S13P (rps13p) CSB-YP311492HTJ
CSB-EP311492HTJ
CSB-BP311492HTJ
CSB-MP311492HTJ
CSB-EP311492HTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydroxyurea phosphotransferase (hur) CSB-YP311493FNW
CSB-EP311493FNW
CSB-BP311493FNW
CSB-MP311493FNW
CSB-EP311493FNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetracycline resistance protein tetQ (tetQ), partial CSB-YP311494BDT
CSB-EP311494BDT
CSB-BP311494BDT
CSB-MP311494BDT
CSB-EP311494BDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyberlindnera saturnus Killer toxin (HSK) CSB-YP311495CVC
CSB-EP311495CVC
CSB-BP311495CVC
CSB-MP311495CVC
CSB-EP311495CVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cauliflower mosaic virus Transactivator/viroplasmin protein (ORF VI), partial CSB-YP311496CFD
CSB-EP311496CFD
CSB-BP311496CFD
CSB-MP311496CFD
CSB-EP311496CFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cauliflower mosaic virus Aphid transmission protein (ORF II) CSB-YP311497CFD
CSB-EP311497CFD
CSB-BP311497CFD
CSB-MP311497CFD
CSB-EP311497CFD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-lactamase OXA-5 (bla) CSB-YP311498EZW
CSB-EP311498EZW
CSB-BP311498EZW
CSB-MP311498EZW
CSB-EP311498EZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus carnosus 50S ribosomal protein L13 (rplM) CSB-YP311499FLK
CSB-EP311499FLK
CSB-BP311499FLK
CSB-MP311499FLK
CSB-EP311499FLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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