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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Beta-carotene hydroxylase (crtZ) CSB-YP311521EOS
CSB-EP311521EOS
CSB-BP311521EOS
CSB-MP311521EOS
CSB-EP311521EOS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 6A Protein U3 (U3) CSB-YP311522HJZ
CSB-EP311522HJZ
CSB-BP311522HJZ
CSB-MP311522HJZ
CSB-EP311522HJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Barley yellow mosaic virus Genome polyprotein 2 (RNA2), partial CSB-YP311523BES
CSB-EP311523BES
CSB-BP311523BES
CSB-MP311523BES
CSB-EP311523BES-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa White collar 1 protein (wc-1), partial CSB-YP311524NHA
CSB-EP311524NHA
CSB-BP311524NHA
CSB-MP311524NHA
CSB-EP311524NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haliotis sorenseni Egg-lysin CSB-YP311525HBC
CSB-EP311525HBC
CSB-BP311525HBC
CSB-MP311525HBC
CSB-EP311525HBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase BCK1/SLK1/SSP31 (BCK1), partial CSB-YP311526SVG
CSB-EP311526SVG
CSB-BP311526SVG
CSB-MP311526SVG
CSB-EP311526SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 1,4-alpha-glucan-branching enzyme, chloroplastic/amyloplastic (SBE1), partial CSB-YP311527OFG
CSB-EP311527OFG
CSB-BP311527OFG
CSB-MP311527OFG
CSB-EP311527OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Src substrate protein p85 (CTTN1), partial CSB-YP311528CH
CSB-EP311528CH
CSB-BP311528CH
CSB-MP311528CH
CSB-EP311528CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Membrane transporter D1, partial CSB-YP311529LOE
CSB-EP311529LOE
CSB-BP311529LOE
CSB-MP311529LOE
CSB-EP311529LOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Rod shape-determining protein mreD (mreD), partial CSB-YP311530BRJ1
CSB-EP311530BRJ1
CSB-BP311530BRJ1
CSB-MP311530BRJ1
CSB-EP311530BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Protein rcaC (rcaC), partial CSB-YP311531FCY
CSB-EP311531FCY
CSB-BP311531FCY
CSB-MP311531FCY
CSB-EP311531FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dhori virus Putative uncharacterized protein M2 (M2) CSB-YP311532DDL
CSB-EP311532DDL
CSB-BP311532DDL
CSB-MP311532DDL
CSB-EP311532DDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Mitochondrial outer membrane protein porin (porA) CSB-YP311533DKK
CSB-EP311533DKK
CSB-BP311533DKK
CSB-MP311533DKK
CSB-EP311533DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Elongation factor 1-alpha (TEF) CSB-YP311534EXI
CSB-EP311534EXI
CSB-BP311534EXI
CSB-MP311534EXI
CSB-EP311534EXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Selaginella martensii Phytochrome 1 (PHY1), partial CSB-YP311535SEP
CSB-EP311535SEP
CSB-BP311535SEP
CSB-MP311535SEP
CSB-EP311535SEP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bufo marinus Lipocalin CSB-YP311536BXI
CSB-EP311536BXI
CSB-BP311536BXI
CSB-MP311536BXI
CSB-EP311536BXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allium sativum Alliin lyase 1 CSB-YP311537AHL
CSB-BP311537AHL
CSB-MP311537AHL
CSB-EP311537AHL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer membrane pore protein E (phoE) CSB-YP311538KBE
CSB-EP311538KBE
CSB-BP311538KBE
CSB-MP311538KBE
CSB-EP311538KBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza C virus Non-structural protein 1 (NS) CSB-YP311539IKB
CSB-EP311539IKB
CSB-BP311539IKB
CSB-MP311539IKB
CSB-EP311539IKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus M 12 kDa protein CSB-YP311540PQZ
CSB-EP311540PQZ
CSB-BP311540PQZ
CSB-MP311540PQZ
CSB-EP311540PQZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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