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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein yddT (yddT) CSB-YP310631BRJ
CSB-EP310631BRJ
CSB-BP310631BRJ
CSB-MP310631BRJ
CSB-EP310631BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydeE (ydeE) CSB-YP310632BRJ
CSB-EP310632BRJ
CSB-BP310632BRJ
CSB-MP310632BRJ
CSB-EP310632BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydeM (ydeM) CSB-YP310633BRJ
CSB-EP310633BRJ
CSB-BP310633BRJ
CSB-MP310633BRJ
CSB-EP310633BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ydfK (ydfK), partial CSB-YP310634BRJ1
CSB-EP310634BRJ1
CSB-BP310634BRJ1
CSB-MP310634BRJ1
CSB-EP310634BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative NAD (P)H nitroreductase ydgI CSB-YP310635BRJ
CSB-EP310635BRJ
CSB-BP310635BRJ
CSB-MP310635BRJ
CSB-EP310635BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ywqM (ywqM) CSB-YP310636BRJ
CSB-EP310636BRJ
CSB-BP310636BRJ
CSB-MP310636BRJ
CSB-EP310636BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis PGA synthase CapB (capB) CSB-YP310637BRJ
CSB-EP310637BRJ
CSB-BP310637BRJ
CSB-MP310637BRJ
CSB-EP310637BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Flagellar hook-associated protein 2 (fliD), partial CSB-YP310638HUV
CSB-EP310638HUV
CSB-BP310638HUV
CSB-MP310638HUV
CSB-EP310638HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris 6-phosphogluconate dehydrogenase, decarboxylating (gnd) CSB-YP310639LND
CSB-EP310639LND
CSB-BP310639LND
CSB-MP310639LND
CSB-EP310639LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative short-chain type dehydrogenase/reductase Rv0148 (Rv0148) CSB-YP310640MVZ
CSB-EP310640MVZ
CSB-BP310640MVZ
CSB-MP310640MVZ
CSB-EP310640MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1 (10),2-diene-4-oate hydrolase CSB-YP310641MVZ
CSB-EP310641MVZ
CSB-BP310641MVZ
CSB-MP310641MVZ
CSB-EP310641MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant dTDP-4-dehydrorhamnose reductase (rmlD) CSB-YP310642MVZ
CSB-EP310642MVZ
CSB-BP310642MVZ
CSB-MP310642MVZ
CSB-EP310642MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0336 protein Rv0635/MT0664 (Rv0635, MT0664) CSB-YP310643MVZ
CSB-EP310643MVZ
CSB-BP310643MVZ
CSB-MP310643MVZ
CSB-EP310643MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa DNA repair protein RadA homolog (radA) CSB-YP310644EZX
CSB-EP310644EZX
CSB-BP310644EZX
CSB-MP310644EZX
CSB-EP310644EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ygaN (ygaN) CSB-YP310645BRJ
CSB-EP310645BRJ
CSB-BP310645BRJ
CSB-MP310645BRJ
CSB-EP310645BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Multidrug resistance ABC transporter ATP-binding and permease protein (lmrA), partial CSB-YP310646LND1
CSB-EP310646LND1
CSB-BP310646LND1
CSB-MP310646LND1
CSB-EP310646LND1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Electron transport complex protein RnfE (rnfE), partial CSB-YP310647RLC1
CSB-EP310647RLC1
CSB-BP310647RLC1
CSB-MP310647RLC1
CSB-EP310647RLC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP310648NGC
CSB-EP310648NGC
CSB-BP310648NGC
CSB-MP310648NGC
CSB-EP310648NGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ysmB (ysmB) CSB-YP310649BRJ
CSB-EP310649BRJ
CSB-BP310649BRJ
CSB-MP310649BRJ
CSB-EP310649BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zinc metalloproteinase dpy-31 (dpy-31), partial CSB-YP310650CXY
CSB-EP310650CXY
CSB-BP310650CXY
CSB-MP310650CXY
CSB-EP310650CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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