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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Putative xylose-proton symporter (xylP), partial CSB-YP311036LAZ
CSB-EP311036LAZ
CSB-BP311036LAZ
CSB-MP311036LAZ
CSB-EP311036LAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant HTH-type transcriptional repressor KstR2 (kstR2) CSB-YP311037MVZ
CSB-EP311037MVZ
CSB-BP311037MVZ
CSB-MP311037MVZ
CSB-EP311037MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative thiosulfate sulfurtransferase sseA (sseA) CSB-YP311038MVZ
CSB-EP311038MVZ
CSB-BP311038MVZ
CSB-MP311038MVZ
CSB-EP311038MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative antitoxin VapB5 (vapB5) CSB-YP311039MVZ
CSB-EP311039MVZ
CSB-BP311039MVZ
CSB-MP311039MVZ
CSB-EP311039MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease V gamma chain (recC), partial CSB-YP311040MVZ
CSB-EP311040MVZ
CSB-BP311040MVZ
CSB-MP311040MVZ
CSB-EP311040MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP311041NBM
CSB-EP311041NBM
CSB-BP311041NBM
CSB-MP311041NBM
CSB-EP311041NBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Outer membrane protein B (ompB), partial CSB-YP311042RNE
CSB-EP311042RNE
CSB-BP311042RNE
CSB-MP311042RNE
CSB-EP311042RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yhbD (yhbD) CSB-YP311043BRJ
CSB-EP311043BRJ
CSB-BP311043BRJ
CSB-MP311043BRJ
CSB-EP311043BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum molischianum Light-harvesting protein B-800/850 alpha chain (A1), partial CSB-YP311045RLZ
CSB-EP311045RLZ
CSB-BP311045RLZ
CSB-MP311045RLZ
CSB-EP311045RLZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 (Inpp5d), partial CSB-YP311046RA
CSB-EP311046RA
CSB-BP311046RA
CSB-MP311046RA
CSB-EP311046RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Mast cell protease 8 (Mcpt8) CSB-YP311047RA
CSB-EP311047RA
CSB-BP311047RA
CSB-MP311047RA
CSB-EP311047RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Complement C3 (c3), partial CSB-YP311048OEI
CSB-EP311048OEI
CSB-BP311048OEI
CSB-MP311048OEI
CSB-EP311048OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Subgroup A Rous sarcoma virus receptor pg950, partial CSB-YP311049DXJ
CSB-EP311049DXJ
CSB-BP311049DXJ
CSB-MP311049DXJ
CSB-EP311049DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Catharanthus roseus Tabersonine 16-hydroxylase (CYP71D12) CSB-YP311050DQN
CSB-EP311050DQN
CSB-BP311050DQN
CSB-MP311050DQN
CSB-EP311050DQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP311051SKY
CSB-EP311051SKY
CSB-BP311051SKY
CSB-MP311051SKY
CSB-EP311051SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Succinyl-CoA ligase [ADP-forming] subunit alpha (sucD) CSB-YP311052SKY
CSB-EP311052SKY
CSB-BP311052SKY
CSB-MP311052SKY
CSB-EP311052SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 60 kDa chaperonin (groL), partial CSB-YP311053SKY
CSB-EP311053SKY
CSB-BP311053SKY
CSB-MP311053SKY
CSB-EP311053SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP311054SKY
CSB-EP311054SKY
CSB-BP311054SKY
CSB-MP311054SKY
CSB-EP311054SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Adenylosuccinate synthetase (purA) CSB-YP311055SKY
CSB-EP311055SKY
CSB-BP311055SKY
CSB-MP311055SKY
CSB-EP311055SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 10 kDa chaperonin (groS) CSB-YP311056SKY
CSB-EP311056SKY
CSB-BP311056SKY
CSB-MP311056SKY
CSB-EP311056SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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