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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Feline coronavirus Spike glycoprotein (S), partial CSB-YP319859FEJ
CSB-BP319859FEJ
CSB-MP319859FEJ
CSB-EP319859FEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homeobox protein mab-5 (mab-5) CSB-YP319860CXY
CSB-EP319860CXY
CSB-BP319860CXY
CSB-MP319860CXY
CSB-EP319860CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carica papaya Caricain CSB-YP319861DQH
CSB-EP319861DQH
CSB-BP319861DQH
CSB-MP319861DQH
CSB-EP319861DQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger protein 27 (Zfp27), partial CSB-YP319862MO
CSB-EP319862MO
CSB-BP319862MO
CSB-MP319862MO
CSB-EP319862MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glyceraldehyde-3-phosphate dehydrogenase, cytosolic CSB-YP319863TQV
CSB-EP319863TQV
CSB-BP319863TQV
CSB-MP319863TQV
CSB-EP319863TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum 30S ribosomal protein S17 (rpsQ) CSB-YP319864MLJ
CSB-EP319864MLJ
CSB-BP319864MLJ
CSB-MP319864MLJ
CSB-EP319864MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum 50S ribosomal protein L4 (rplD) CSB-YP319865MLJ
CSB-EP319865MLJ
CSB-BP319865MLJ
CSB-MP319865MLJ
CSB-EP319865MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum 50S ribosomal protein L24 (rplX) CSB-YP319866MLJ
CSB-EP319866MLJ
CSB-BP319866MLJ
CSB-MP319866MLJ
CSB-EP319866MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutaminase-asparaginase (ansB) CSB-YP319867ABW
CSB-EP319867ABW
CSB-BP319867ABW
CSB-MP319867ABW
CSB-EP319867ABW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Nuclear protein UL4 (UL4) CSB-YP319868HWY
CSB-EP319868HWY
CSB-BP319868HWY
CSB-MP319868HWY
CSB-EP319868HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Replication origin-binding protein (UL9), partial CSB-YP319869HWY
CSB-EP319869HWY
CSB-BP319869HWY
CSB-MP319869HWY
CSB-EP319869HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Protein UL20 (UL20), partial CSB-YP319870HWY1
CSB-EP319870HWY1
CSB-BP319870HWY1
CSB-MP319870HWY1
CSB-EP319870HWY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Protease precursor (UL26), partial CSB-YP319871HWY
CSB-EP319871HWY
CSB-BP319871HWY
CSB-MP319871HWY
CSB-EP319871HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Virion egress protein UL34 (UL34), partial CSB-YP319872HWY1
CSB-EP319872HWY1
CSB-BP319872HWY1
CSB-MP319872HWY1
CSB-EP319872HWY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Capsid assembly protein UL37 (UL37), partial CSB-YP319873HWY
CSB-EP319873HWY
CSB-BP319873HWY
CSB-MP319873HWY
CSB-EP319873HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Tegument protein VP22 (UL49) CSB-YP319874HWY
CSB-EP319874HWY
CSB-BP319874HWY
CSB-MP319874HWY
CSB-EP319874HWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodotorula rubra Phenylalanine ammonia-lyase (PAL), partial CSB-YP319875RED
CSB-EP319875RED
CSB-BP319875RED
CSB-MP319875RED
CSB-EP319875RED-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus keta Gonadotropin subunit beta-1 (cgba) CSB-YP319876OBG
CSB-EP319876OBG
CSB-BP319876OBG
CSB-MP319876OBG
CSB-EP319876OBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse mammary tumor virus Envelope glycoprotein gp70 (env), partial CSB-YP319877MIT
CSB-EP319877MIT
CSB-BP319877MIT
CSB-MP319877MIT
CSB-EP319877MIT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pol polyprotein (pol) CSB-YP319878FAN
CSB-EP319878FAN
CSB-BP319878FAN
CSB-MP319878FAN
CSB-EP319878FAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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