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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Heat shock 70-related protein 1, mitochondrial (HSP70.1), partial CSB-YP320063LOG
CSB-EP320063LOG
CSB-BP320063LOG
CSB-MP320063LOG
CSB-EP320063LOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pachycara brachycephalum Ice-structuring protein AB2 CSB-YP320064PAB
CSB-EP320064PAB
CSB-BP320064PAB
CSB-MP320064PAB
CSB-EP320064PAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP320065MCB
CSB-EP320065MCB
CSB-BP320065MCB
CSB-MP320065MCB
CSB-EP320065MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Translation initiation factor IF-1, chloroplastic (infA) CSB-YP320066MCB
CSB-EP320066MCB
CSB-BP320066MCB
CSB-MP320066MCB
CSB-EP320066MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 30S ribosomal protein S12, chloroplastic (rps12-A) CSB-YP320067OFG
CSB-EP320067OFG
CSB-BP320067OFG
CSB-MP320067OFG
CSB-EP320067OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Photosystem I P700 chlorophyll a apoprotein A1 (psaA), partial CSB-YP320068DSJ
CSB-EP320068DSJ
CSB-BP320068DSJ
CSB-MP320068DSJ
CSB-EP320068DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Maturase K (matK) CSB-YP320069MCB
CSB-EP320069MCB
CSB-BP320069MCB
CSB-MP320069MCB
CSB-EP320069MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Photosystem II reaction center protein T (psbT), partial CSB-YP320070MCB
CSB-EP320070MCB
CSB-BP320070MCB
CSB-MP320070MCB
CSB-EP320070MCB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Photosystem I reaction center subunit VIII (psaI), partial CSB-YP320071OFG
CSB-EP320071OFG
CSB-BP320071OFG
CSB-MP320071OFG
CSB-EP320071OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Photosystem I reaction center subunit IX (psaJ), partial CSB-YP320072NHE
CSB-EP320072NHE
CSB-BP320072NHE
CSB-MP320072NHE
CSB-EP320072NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum 50S ribosomal protein L32, chloroplastic (rpl32) CSB-YP320073NHE
CSB-EP320073NHE
CSB-BP320073NHE
CSB-MP320073NHE
CSB-EP320073NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Photosystem I assembly protein ycf3 (ycf3) CSB-YP320074NHE
CSB-EP320074NHE
CSB-BP320074NHE
CSB-MP320074NHE
CSB-EP320074NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP320075NHE
CSB-EP320075NHE
CSB-BP320075NHE
CSB-MP320075NHE
CSB-EP320075NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta, chloroplastic (accD) CSB-YP320076NHE
CSB-EP320076NHE
CSB-BP320076NHE
CSB-MP320076NHE
CSB-EP320076NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Fibroblast growth factor 2 (fgf2) CSB-YP320077XBE
CSB-EP320077XBE
CSB-BP320077XBE
CSB-MP320077XBE
CSB-EP320077XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequence element IS231C CSB-YP320078BCW
CSB-EP320078BCW
CSB-BP320078BCW
CSB-MP320078BCW
CSB-EP320078BCW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Transposable element Hobo transposase (T), partial CSB-YP320079DLU
CSB-EP320079DLU
CSB-BP320079DLU
CSB-MP320079DLU
CSB-EP320079DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Arylamine N-acetyltransferase, liver isozyme CSB-YP320080CH
CSB-EP320080CH
CSB-BP320080CH
CSB-MP320080CH
CSB-EP320080CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Protein usg (usg) CSB-YP320081DQO
CSB-EP320081DQO
CSB-BP320081DQO
CSB-MP320081DQO
CSB-EP320081DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Endonuclease SceI small subunit (ENS2), partial CSB-YP320082SAC
CSB-EP320082SAC
CSB-BP320082SAC
CSB-MP320082SAC
CSB-EP320082SAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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