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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Candida tropicalis Acyl-coenzyme A oxidase 2 (POX2), partial CSB-YP320861CZN
CSB-EP320861CZN
CSB-BP320861CZN
CSB-MP320861CZN
CSB-EP320861CZN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP320862TQN
CSB-EP320862TQN
CSB-BP320862TQN
CSB-MP320862TQN
CSB-EP320862TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. R-phycocyanin-2 subunit alpha (rpcA) CSB-YP320863SSM
CSB-EP320863SSM
CSB-BP320863SSM
CSB-MP320863SSM
CSB-EP320863SSM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mastigocladus laminosus Phycobilisome 34.5 kDa linker polypeptide, phycoerythrocyanin-associated, rod (pecC) CSB-YP320864MQJ
CSB-EP320864MQJ
CSB-BP320864MQJ
CSB-MP320864MQJ
CSB-EP320864MQJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea ATP synthase delta chain, chloroplastic (ATPD) CSB-YP320865FKI
CSB-EP320865FKI
CSB-BP320865FKI
CSB-MP320865FKI
CSB-EP320865FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein RecA (recA) CSB-YP320866EYY
CSB-EP320866EYY
CSB-BP320866EYY
CSB-MP320866EYY
CSB-EP320866EYY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase MspI (mspIM) CSB-YP320867MID
CSB-EP320867MID
CSB-BP320867MID
CSB-MP320867MID
CSB-EP320867MID-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein daughterless (da), partial CSB-YP320868DLU
CSB-EP320868DLU
CSB-BP320868DLU
CSB-MP320868DLU
CSB-EP320868DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cell division control protein 24 (CDC24), partial CSB-YP320869SVG
CSB-EP320869SVG
CSB-BP320869SVG
CSB-MP320869SVG
CSB-EP320869SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas savastanoi pv. glycinea Avirulence protein A (avrA), partial CSB-YP320870FGM
CSB-EP320870FGM
CSB-BP320870FGM
CSB-MP320870FGM
CSB-EP320870FGM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Vitelline membrane protein Vm26Aa (Vm26Aa) CSB-YP320871DLU
CSB-EP320871DLU
CSB-BP320871DLU
CSB-MP320871DLU
CSB-EP320871DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Chloromuconate cycloisomerase (clcB) CSB-YP320872FFZ
CSB-EP320872FFZ
CSB-BP320872FFZ
CSB-MP320872FFZ
CSB-EP320872FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhoptry antigen protein CSB-YP320873PLO
CSB-EP320873PLO
CSB-BP320873PLO
CSB-MP320873PLO
CSB-EP320873PLO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ysmA (ysmA) CSB-YP320874BRJ
CSB-EP320874BRJ
CSB-BP320874BRJ
CSB-MP320874BRJ
CSB-EP320874BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Heat shock protein SSB1 (SSB1), partial CSB-YP320875SVG
CSB-EP320875SVG
CSB-BP320875SVG
CSB-MP320875SVG
CSB-EP320875SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-YP320876ILK
CSB-EP320876ILK
CSB-BP320876ILK
CSB-MP320876ILK
CSB-EP320876ILK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anemonia sulcata Delta/kappa-actitoxin-Avd4a CSB-YP320877AKE
CSB-EP320877AKE
CSB-BP320877AKE
CSB-MP320877AKE
CSB-EP320877AKE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactose-specific phosphotransferase enzyme IIA component (lacF) CSB-YP320878LMH
CSB-EP320878LMH
CSB-BP320878LMH
CSB-MP320878LMH
CSB-EP320878LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus acidocaldarius DNA-directed RNA polymerase subunit H (rpoH) CSB-YP320880FOZ
CSB-EP320880FOZ
CSB-BP320880FOZ
CSB-MP320880FOZ
CSB-EP320880FOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phanerodontia chrysosporium Ligninase H2 (GLG4) CSB-YP320881EUK
CSB-BP320881EUK
CSB-MP320881EUK
CSB-EP320881EUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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