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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana G-type lectin S-receptor-like serine/threonine-protein kinase SRK (SRK), partial CSB-YP319837DOA
CSB-EP319837DOA
CSB-BP319837DOA
CSB-MP319837DOA
CSB-EP319837DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At1g25150 (At1g25150) CSB-YP319838DOA
CSB-EP319838DOA
CSB-BP319838DOA
CSB-MP319838DOA
CSB-EP319838DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops marajoensis Inactive phospholipase A2 CSB-YP319839BUY
CSB-EP319839BUY
CSB-BP319839BUY
CSB-MP319839BUY
CSB-EP319839BUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porin omp2b (omp2b) CSB-YP319840BMP
CSB-EP319840BMP
CSB-BP319840BMP
CSB-MP319840BMP
CSB-EP319840BMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Candidapepsin-2 (SAP2) CSB-YP319841CZD
CSB-EP319841CZD
CSB-BP319841CZD
CSB-MP319841CZD
CSB-EP319841CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 30S ribosomal protein S3 (rpsC) CSB-YP319842MEO
CSB-EP319842MEO
CSB-BP319842MEO
CSB-MP319842MEO
CSB-EP319842MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anguilla japonica Antimicrobial peptide AJN-10 CSB-YP319843BZH
CSB-EP319843BZH
CSB-BP319843BZH
CSB-MP319843BZH
CSB-EP319843BZH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaejovis mexicanus smithi Potassium channel toxin alpha-KTx 21.2 CSB-YP319844VAK
CSB-EP319844VAK
CSB-BP319844VAK
CSB-MP319844VAK
CSB-EP319844VAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum HNTX-03141017 CSB-YP319845HUK
CSB-EP319845HUK
CSB-BP319845HUK
CSB-MP319845HUK
CSB-EP319845HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haplopelma hainanum HNTX-031496134 CSB-YP319846HUK
CSB-EP319846HUK
CSB-BP319846HUK
CSB-MP319846HUK
CSB-EP319846HUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops leucurus Zinc metalloproteinase-disintegrin leucurogin CSB-YP319847BIY
CSB-EP319847BIY
CSB-BP319847BIY
CSB-MP319847BIY
CSB-EP319847BIY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus californicus Conotoxin Cltx-4 CSB-YP319848DVU
CSB-EP319848DVU
CSB-BP319848DVU
CSB-MP319848DVU
CSB-EP319848DVU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus quercinus Conotoxin Qc5.1 CSB-YP319849CXE
CSB-EP319849CXE
CSB-BP319849CXE
CSB-MP319849CXE
CSB-EP319849CXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops asper Snaclec aspercetin subunit alpha CSB-YP319850BUT
CSB-EP319850BUT
CSB-BP319850BUT
CSB-MP319850BUT
CSB-EP319850BUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RANBP2-like and GRIP domain-containing protein 2 (RGPD2), partial CSB-YP319851HU
CSB-EP319851HU
CSB-BP319851HU
CSB-MP319851HU
CSB-EP319851HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latrodectus hesperus Delta-latroinsectotoxin-Lhe1a CSB-YP319853LDD
CSB-EP319853LDD
CSB-BP319853LDD
CSB-MP319853LDD
CSB-EP319853LDD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Protein PET117 homolog, mitochondrial (Pet117) CSB-YP319854MO
CSB-EP319854MO
CSB-BP319854MO
CSB-MP319854MO
CSB-EP319854MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 14.7 kDa protein CSB-YP319856ENL
CSB-EP319856ENL
CSB-BP319856ENL
CSB-MP319856ENL
CSB-EP319856ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized 30.3 kDa protein CSB-YP319857BRN
CSB-EP319857BRN
CSB-BP319857BRN
CSB-MP319857BRN
CSB-EP319857BRN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Relaxosome protein TraM (traM) CSB-YP319858ENV
CSB-EP319858ENV
CSB-BP319858ENV
CSB-MP319858ENV
CSB-EP319858ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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