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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Escherichia coli Arginine--tRNA ligase (argS) CSB-YP320042ENV
CSB-EP320042ENV
CSB-BP320042ENV
CSB-MP320042ENV
CSB-EP320042ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2-enoate reductase CSB-YP320043CMP
CSB-EP320043CMP
CSB-BP320043CMP
CSB-MP320043CMP
CSB-EP320043CMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chenopodium rubrum Small heat shock protein, chloroplastic (HSP23), partial CSB-YP320044DRT
CSB-EP320044DRT
CSB-BP320044DRT
CSB-MP320044DRT
CSB-EP320044DRT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase for insertion sequence element IS421 CSB-YP320045ENL
CSB-EP320045ENL
CSB-BP320045ENL
CSB-MP320045ENL
CSB-EP320045ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein stbB (stbB) CSB-YP320046ENL
CSB-EP320046ENL
CSB-BP320046ENL
CSB-MP320046ENL
CSB-EP320046ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria gonorrhoeae Outer membrane protein H.8 (NGO0983) CSB-YP320047NGA
CSB-EP320047NGA
CSB-BP320047NGA
CSB-MP320047NGA
CSB-EP320047NGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eleginus gracilis Ice-structuring glycoprotein 8R CSB-YP320048EBG
CSB-EP320048EBG
CSB-BP320048EBG
CSB-MP320048EBG
CSB-EP320048EBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Fimbrial protein (fimA) CSB-YP320050DDR
CSB-EP320050DDR
CSB-BP320050DDR
CSB-MP320050DDR
CSB-EP320050DDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Metallothionein-2 (MtnB) CSB-YP320051DLU
CSB-EP320051DLU
CSB-BP320051DLU
CSB-MP320051DLU
CSB-EP320051DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum Lignin-forming anionic peroxidase CSB-YP320052NHE
CSB-EP320052NHE
CSB-BP320052NHE
CSB-MP320052NHE
CSB-EP320052NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bryonia dioica Trypsin inhibitor 2 CSB-YP320053BMR
CSB-EP320053BMR
CSB-BP320053BMR
CSB-MP320053BMR
CSB-EP320053BMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cardisoma carnifex Neuropeptide H CSB-YP320054CDT
CSB-EP320054CDT
CSB-BP320054CDT
CSB-MP320054CDT
CSB-EP320054CDT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus 50 kDa spicule matrix protein (SM50) CSB-YP320055FOT
CSB-EP320055FOT
CSB-BP320055FOT
CSB-MP320055FOT
CSB-EP320055FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP320056BRJ
CSB-EP320056BRJ
CSB-BP320056BRJ
CSB-MP320056BRJ
CSB-EP320056BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Gluconate permease (gntP), partial CSB-YP320057BRJ
CSB-EP320057BRJ
CSB-BP320057BRJ
CSB-MP320057BRJ
CSB-EP320057BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus oreganus concolor Myotoxin-2 CSB-YP320058DYF
CSB-EP320058DYF
CSB-BP320058DYF
CSB-MP320058DYF
CSB-EP320058DYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication initiation protein (repE) CSB-YP320059FKZ
CSB-EP320059FKZ
CSB-BP320059FKZ
CSB-MP320059FKZ
CSB-EP320059FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloramphenicol resistance protein (cml) CSB-YP320060ENL
CSB-EP320060ENL
CSB-BP320060ENL
CSB-MP320060ENL
CSB-EP320060ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fimbrial protein (sefA) CSB-YP320061SBG
CSB-EP320061SBG
CSB-BP320061SBG
CSB-MP320061SBG
CSB-EP320061SBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Lysozyme C-3 CSB-YP320062PI
CSB-EP320062PI
CSB-BP320062PI
CSB-MP320062PI
CSB-EP320062PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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