Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Enterobacteria phage T4 Uncharacterized 7.1 kDa protein in tk-vs intergenic region (y06B) CSB-YP321089EDZ
CSB-EP321089EDZ
CSB-BP321089EDZ
CSB-MP321089EDZ
CSB-EP321089EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 10.2 kDa protein in regB-denV intergenic region (y06G) CSB-YP321090EDZ
CSB-EP321090EDZ
CSB-BP321090EDZ
CSB-MP321090EDZ
CSB-EP321090EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Uncharacterized 12.8 kDa protein in regB-denV intergenic region (y06J) CSB-YP321091EDZ
CSB-EP321091EDZ
CSB-BP321091EDZ
CSB-MP321091EDZ
CSB-EP321091EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Double-stranded DNA-binding protein (dsbA) CSB-YP321092EDZ
CSB-BP321092EDZ
CSB-MP321092EDZ
CSB-EP321092EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Putative endonuclease segB (segB) CSB-YP321093EDZ
CSB-EP321093EDZ
CSB-BP321093EDZ
CSB-MP321093EDZ
CSB-EP321093EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Tail sheath-stabilizing protein Gp3 (3) CSB-YP321094EDZ
CSB-EP321094EDZ
CSB-BP321094EDZ
CSB-MP321094EDZ
CSB-EP321094EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Tail tube protein Gp19 (19) CSB-YP321095EDZ
CSB-EP321095EDZ
CSB-BP321095EDZ
CSB-MP321095EDZ
CSB-EP321095EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Tail-tube assembly protein Gp29 (29), partial CSB-YP321096EDZ
CSB-EP321096EDZ
CSB-BP321096EDZ
CSB-MP321096EDZ
CSB-EP321096EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage T4 Tail-tube assembly protein Gp54 (54) CSB-YP321097EDZ
CSB-EP321097EDZ
CSB-BP321097EDZ
CSB-MP321097EDZ
CSB-EP321097EDZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lysis protein for colicin E6 (lys) CSB-YP321098ENL
CSB-EP321098ENL
CSB-BP321098ENL
CSB-MP321098ENL
CSB-EP321098ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Photosystem I reaction center subunit VI, chloroplastic (PSAH), partial CSB-YP321099DSJ1
CSB-EP321099DSJ1
CSB-BP321099DSJ1
CSB-MP321099DSJ1
CSB-EP321099DSJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein glass (gl), partial CSB-YP321100DLU
CSB-EP321100DLU
CSB-BP321100DLU
CSB-MP321100DLU
CSB-EP321100DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida DNA gyrase subunit B (gyrB), partial CSB-YP321101FFZ
CSB-EP321101FFZ
CSB-BP321101FFZ
CSB-MP321101FFZ
CSB-EP321101FFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein sevenless (sev), partial CSB-YP321102DLU
CSB-EP321102DLU
CSB-BP321102DLU
CSB-MP321102DLU
CSB-EP321102DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Glucose-6-phosphate isomerase B (pgiB) CSB-YP321103GFM
CSB-EP321103GFM
CSB-BP321103GFM
CSB-MP321103GFM
CSB-EP321103GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit A (POL1), partial CSB-YP321104SVG
CSB-EP321104SVG
CSB-BP321104SVG
CSB-MP321104SVG
CSB-EP321104SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sucrose-6-phosphate hydrolase (scrB) CSB-YP321105VEU
CSB-EP321105VEU
CSB-BP321105VEU
CSB-MP321105VEU
CSB-EP321105VEU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium sp. 6-aminohexanoate-cyclic-dimer hydrolase (nylA) CSB-YP321106FBV
CSB-EP321106FBV
CSB-BP321106FBV
CSB-MP321106FBV
CSB-EP321106FBV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma japonicum Immunogenic miracidial antigen 5D (5D) CSB-YP321107SXP
CSB-EP321107SXP
CSB-BP321107SXP
CSB-MP321107SXP
CSB-EP321107SXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricket paralysis virus Structural polyprotein, partial CSB-YP321108CSA
CSB-EP321108CSA
CSB-BP321108CSA
CSB-MP321108CSA
CSB-EP321108CSA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>